Structure of spinach nitrite reductase: Implications for multi-electron reactions by the iron-sulfur:siroheme cofactor

Uma Swamy, Meitian Wang, Jatinda N. Tripathy, Sung Kun Kim, Masakazu Hirasawa, David B. Knaff, James Paul Allen

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The structure of nitrite reductase, a key enzyme in the process of nitrogen assimilation, has been determined using X-ray diffraction to a resolution limit of 2.8 Å. The protein has a globular fold consisting of 3 α/β domains with the siroheme-iron sulfur cofactor at the interface of the three domains. The Fe4S4 cluster is coordinated by cysteines 441, 447, 482, and 486. The siroheme is located at a distance of 4.2 Å from the cluster, and the central iron atom is coordinated to Cys 486. The siroheme is surrounded by several ionizable amino acid residues that facilitate the binding and subsequent reduction of nitrite. A model for the ferredoxin:nitrite reductase complex is proposed in which the binding of ferredoxin to a positively charged region of nitrite reductase results in elimination of exposure of the cofactors to the solvent. The structure of nitrite reductase shows a broad similarity to the hemoprotein subunit of sulfite reductase but has many significant differences in the backbone positions that could reflect sequence differences or could arise from alterations of the sulfite reductase structure that arise from the isolation of this subunit from the native complex. The implications of the nitrite reductase structure for understanding multi-electron processes are discussed in terms of differences in the protein environments of the cofactors.

Original languageEnglish
Pages (from-to)16054-16063
Number of pages10
JournalBiochemistry
Volume44
Issue number49
DOIs
Publication statusPublished - Dec 13 2005

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Nitrite Reductases
Spinacia oleracea
Sulfur
Oxidoreductases Acting on Sulfur Group Donors
Iron
Electrons
Ferredoxin-Nitrite Reductase
Ferredoxins
Nitrites
X-Ray Diffraction
Cysteine
Proteins
Nitrogen
Amino Acids
X ray diffraction
Atoms
siroheme
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structure of spinach nitrite reductase : Implications for multi-electron reactions by the iron-sulfur:siroheme cofactor. / Swamy, Uma; Wang, Meitian; Tripathy, Jatinda N.; Kim, Sung Kun; Hirasawa, Masakazu; Knaff, David B.; Allen, James Paul.

In: Biochemistry, Vol. 44, No. 49, 13.12.2005, p. 16054-16063.

Research output: Contribution to journalArticle

Swamy, Uma ; Wang, Meitian ; Tripathy, Jatinda N. ; Kim, Sung Kun ; Hirasawa, Masakazu ; Knaff, David B. ; Allen, James Paul. / Structure of spinach nitrite reductase : Implications for multi-electron reactions by the iron-sulfur:siroheme cofactor. In: Biochemistry. 2005 ; Vol. 44, No. 49. pp. 16054-16063.
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