Structures of proteins and cofactors: X-ray crystallography

James Paul Allen, C. Seng, C. Larson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Protein crystallography is the predominately used technique for the determination of the three-dimensional structures of proteins and other macromolecules. In this article, the methodology utilized for the measurement and analysis of the diffraction data from crystals is briefly reviewed. As examples of both the usefulness and difficulties of this technique, the determination of the structures of several photosynthetic pigment-protein complexes is described, namely, the reaction center from purple bacteria, photosystem I and photosystem II from cyanobacteria, the light-harvesting complex II from purple bacteria, and the FMO protein from green bacteria.

Original languageEnglish
Pages (from-to)231-240
Number of pages10
JournalPhotosynthesis Research
Volume102
Issue number2
DOIs
Publication statusPublished - Nov 2009

Fingerprint

X ray crystallography
X Ray Crystallography
protein structure
X-ray diffraction
Proteobacteria
crystallography
Photosystem I Protein Complex
Crystallography
Proteins
Photosystem II Protein Complex
light harvesting complex
proteins
photosystem I
Cyanobacteria
Macromolecules
Pigments
photosystem II
crystals
Bacteria
Diffraction

Keywords

  • Light-harvesting complexes
  • Photosystem I
  • Photosystem II
  • Protein crystallography
  • Reaction centers
  • X-ray diffraction

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Biochemistry

Cite this

Structures of proteins and cofactors : X-ray crystallography. / Allen, James Paul; Seng, C.; Larson, C.

In: Photosynthesis Research, Vol. 102, No. 2, 11.2009, p. 231-240.

Research output: Contribution to journalArticle

Allen, James Paul ; Seng, C. ; Larson, C. / Structures of proteins and cofactors : X-ray crystallography. In: Photosynthesis Research. 2009 ; Vol. 102, No. 2. pp. 231-240.
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