Sub-diffraction limited imaging with fluorophores exhibiting emission depletion upon saturation

Assaf Avidan, Dan Oron

Research output: Contribution to journalArticle

Abstract

We present a simplified single-beam scheme for depletion-based sub-diffraction-limited imaging which allows for less restrictive illumination conditions. This is done by introducing the concept of fluorophores exhibiting emission depletion upon saturation. We discuss the circumstances under which such a depletion based process is possible, and derive the scaling of the spatial resolution utilizing this scheme. Next, we analyze the proper illumination conditions both in space and time required for sub diffraction limited imaging, and show that it is applicable only under pulsed excitation. Finally, our scheme's advantages and shortcomings relative to alternative realizations of depletion-based sub-diffraction-limited microscopy are discussed.

Original languageEnglish
Pages (from-to)963-969
Number of pages7
JournalOptics Express
Volume17
Issue number2
DOIs
Publication statusPublished - Jan 19 2009

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depletion
saturation
diffraction
illumination
spatial resolution
microscopy
scaling
excitation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Sub-diffraction limited imaging with fluorophores exhibiting emission depletion upon saturation. / Avidan, Assaf; Oron, Dan.

In: Optics Express, Vol. 17, No. 2, 19.01.2009, p. 963-969.

Research output: Contribution to journalArticle

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