Sub-picosecond measurements of primary electron transfer in Rhodopseudomonas viridis reaction centers using near-infrared excitation

Michael R Wasielewski, David M. Tiede

Research output: Contribution to journalArticle

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Abstract

Rate constants for the primary photoinduced electron transfer reactions within the reaction center protein of the photosynthetic bacterium Rhodopseudomonas viridis have been measured using transient optical absorption spectroscopy following excitation of the primary donor P960 directly with 950 nm laser flashes. The time resolution of the experiments was 0.45 ps. The reduction of BPheo b as indicated by absorption changes at 543 nm exhibits monophasic kinetics with a 1.7 × 1011 s1̄ rate constant following direct excitation of P960. Spectral changes at 805 and 835 nm due to perturbations of the intermediary BChl b occur synchronously with those at 543 nm. There is no evidence for formation of BChl b- as a distinct chemical intermediate preceding the reduction of BPheo b.

Original languageEnglish
Pages (from-to)368-372
Number of pages5
JournalFEBS Letters
Volume204
Issue number2
DOIs
Publication statusPublished - Aug 18 1986

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Rhodopseudomonas
Rate constants
Electrons
Photosynthetic Reaction Center Complex Proteins
Infrared radiation
Absorption spectroscopy
Light absorption
Spectrum Analysis
Bacteria
Lasers
Kinetics
Proteins
Experiments

Keywords

  • Bacterial photosynthesis Charge separation Ultrafast spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sub-picosecond measurements of primary electron transfer in Rhodopseudomonas viridis reaction centers using near-infrared excitation. / Wasielewski, Michael R; Tiede, David M.

In: FEBS Letters, Vol. 204, No. 2, 18.08.1986, p. 368-372.

Research output: Contribution to journalArticle

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