TY - JOUR
T1 - Sub-second hyper-spectral low-frequency vibrational imaging via impulsive Raman excitation
AU - Raanan, Dekel
AU - Audier, Xavier
AU - Shivkumar, Siddarth
AU - Asher, Maor
AU - Menahem, Matan
AU - Yaffe, Omer
AU - Forget, Nicolas
AU - Rigneault, Hervé
AU - Oron, Dan
N1 - Funding Information:
Funding. Centre National de la Recherche Scientifique (A-M-AAP-ID-17-13-170228-15.22-RIGNEAULT, ANR-11-IDEX-0001-02); ANR grants France Bio Imaging (ANR-10-INSB-04-01); France Life Imaging (ANR-11-INSB-0006); Plan cancer INSERM (18CP128-00, PC201508); Israeli Centers for Research Excellence; Israeli Ministry of Science Tashtiot Program (712845); CNRS-Weizmann ImagiNano European Associated Laboratory.
Funding Information:
Centre National de la Recherche Scientifique (A-M-AAP-ID-17-13-170228- 15.22-RIGNEAULT, ANR-11-IDEX-0001-02); ANR grants France Bio Imaging (ANR- 10-INSB-04-01); France Life Imaging (ANR-11-INSB-0006); Plan cancer INSERM (18CP128-00, PC201508); Israeli Centers for Research Excellence; Israeli Ministry of Science Tashtiot Program (712845); CNRS-Weizmann ImagiNano European Associated Laboratory. The authors would like to thank Yahel Soffer for the sample preparation.
PY - 2019/11/1
Y1 - 2019/11/1
N2 - Real-time vibrational microscopy has been recently demonstrated by various techniques, most of them utilizing the well-known schemes of coherent anti-stokes Raman scattering and stimulated Raman scattering. These techniques readily provide valuable chemical information mostly in the higher vibrational frequency regime (>400 cm−1). Addressing the low vibrational frequency regime (<200 cm−1) is challenging due to the usage of spectral filters that are required to isolate the signal from the Rayleigh scattered excitation field. In this Letter, we report on rapid, high-resolution, low-frequency (<130 cm−1) vibrational microscopy using impulsive coherent Raman excitation. By combining impulsive excitation with a fast acousto-optic delay line, we detect the Raman-induced optical Kerr lensing and spectral shift effects with a 25 μs pixel dwell time to produce shot-noise limited, low-frequency hyper-spectral images of various samples.
AB - Real-time vibrational microscopy has been recently demonstrated by various techniques, most of them utilizing the well-known schemes of coherent anti-stokes Raman scattering and stimulated Raman scattering. These techniques readily provide valuable chemical information mostly in the higher vibrational frequency regime (>400 cm−1). Addressing the low vibrational frequency regime (<200 cm−1) is challenging due to the usage of spectral filters that are required to isolate the signal from the Rayleigh scattered excitation field. In this Letter, we report on rapid, high-resolution, low-frequency (<130 cm−1) vibrational microscopy using impulsive coherent Raman excitation. By combining impulsive excitation with a fast acousto-optic delay line, we detect the Raman-induced optical Kerr lensing and spectral shift effects with a 25 μs pixel dwell time to produce shot-noise limited, low-frequency hyper-spectral images of various samples.
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U2 - 10.1364/OL.44.005153
DO - 10.1364/OL.44.005153
M3 - Article
C2 - 31674954
AN - SCOPUS:85074347489
VL - 44
SP - 5153
EP - 5156
JO - Optics Letters
JF - Optics Letters
SN - 0146-9592
IS - 21
ER -