Super-resolution microscopy reveals structural diversity in molecular exchange among peptide amphiphile nanofibres

Ricardo M P Da Silva, Daan Van Der Zwaag, Lorenzo Albertazzi, Sungsoo S. Lee, E. W. Meijer, Samuel I Stupp

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The dynamic behaviour of supramolecular systems is an important dimension of their potential functions. Here, we report on the use of stochastic optical reconstruction microscopy to study the molecular exchange of peptide amphiphile nanofibres, supramolecular systems known to have important biomedical functions. Solutions of nanofibres labelled with different dyes (Cy3 and Cy5) were mixed, and the distribution of dyes inserting into initially single-colour nanofibres was quantified using correlative image analysis. Our observations are consistent with an exchange mechanism involving monomers or small clusters of molecules inserting randomly into a fibre. Different exchange rates are observed within the same fibre, suggesting that local cohesive structures exist on the basis of β-sheet discontinuous domains. The results reported here show that peptide amphiphile supramolecular systems can be dynamic and that their intermolecular interactions affect exchange patterns. This information can be used to generate useful aggregate morphologies for improved biomedical function.

Original languageEnglish
Article number11561
JournalNature Communications
Volume7
DOIs
Publication statusPublished - May 19 2016

Fingerprint

Nanofibers
Amphiphiles
peptides
Microscopy
Microscopic examination
microscopy
Peptides
dyes
fibers
Fibers
Exchange interactions
image analysis
Image analysis
Coloring Agents
Color
monomers
Monomers
color
Molecules
molecules

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Super-resolution microscopy reveals structural diversity in molecular exchange among peptide amphiphile nanofibres. / Da Silva, Ricardo M P; Van Der Zwaag, Daan; Albertazzi, Lorenzo; Lee, Sungsoo S.; Meijer, E. W.; Stupp, Samuel I.

In: Nature Communications, Vol. 7, 11561, 19.05.2016.

Research output: Contribution to journalArticle

Da Silva, Ricardo M P ; Van Der Zwaag, Daan ; Albertazzi, Lorenzo ; Lee, Sungsoo S. ; Meijer, E. W. ; Stupp, Samuel I. / Super-resolution microscopy reveals structural diversity in molecular exchange among peptide amphiphile nanofibres. In: Nature Communications. 2016 ; Vol. 7.
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