Supramolecular design of self-assembling nanofibers for cartilage regeneration

Ramille N. Shah, Nirav A. Shah, Marc M.Del Rosario Lim, Caleb Hsieh, Gordon Nuber, Samuel I. Stupp

Research output: Contribution to journalArticle

356 Citations (Scopus)

Abstract

Molecular and supramolecular design of bioactive biomaterials could have a significant impact on regenerative medicine. Ideal regenerative therapies should be minimally invasive, and thus the notion of self-assembling biomaterials programmed to transform from injectable liquids to solid bioactive structures in tissue is highly attractive for clinical translation. We report here on a coassembly system of peptide amphiphile (PA) molecules designed to form nanofibers for cartilage regeneration by displaying a high density of binding epitopes to transforming growth factor β-1 (TGFβ-1). Growth factor release studies showed that passive release of TGFβ-1 was slower from PA gels containing the growth factor binding sites. In vitro experiments indicate these materials support the survival and promote the chondrogenic differentiation of human mesenchymal stem cells. We also show that these materials can promote regeneration of articular cartilage in a full thickness chondral defect treated with microfracture in a rabbit model with or even without the addition of exogenous growth factor. These results demonstrate the potential of a completely synthetic bioactive biomaterial as a therapy to promote cartilage regeneration.

Original languageEnglish
Pages (from-to)3293-3298
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number8
DOIs
Publication statusPublished - Feb 23 2010

    Fingerprint

Keywords

  • Chondral defects
  • Microfracture
  • Peptide amphiphiles
  • Self-assembling biomaterials
  • Transforming growth factor

ASJC Scopus subject areas

  • General

Cite this