Surface Engineered Polymersomes for Enhanced Modulation of Dendritic Cells During Cardiovascular Immunotherapy

Sijia Yi, Xiaohan Zhang, M. Hussain Sangji, Yugang Liu, Sean D. Allen, Baixue Xiao, Sharan Bobbala, Cameron L. Braverman, Lei Cai, Peter I. Hecker, Matthew DeBerge, Edward B. Thorp, Ryan E. Temel, Samuel I Stupp, Evan A. Scott

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The principle cause of cardiovascular disease (CVD) is atherosclerosis, a chronic inflammatory condition characterized by immunologically complex fatty lesions within the intima of arterial vessel walls. Dendritic cells (DCs) are key regulators of atherosclerotic inflammation, with mature DCs generating pro-inflammatory signals within vascular lesions and tolerogenic DCs eliciting atheroprotective cytokine profiles and regulatory T-cell (Treg) activation. Here, the surface chemistry and morphology of synthetic nanocarriers composed of poly(ethylene glycol)-b-poly(propylene sulfide) copolymers to enhance the targeted modulation of DCs by transporting the anti-inflammatory agent 1,25-dihydroxyvitamin D3-(aVD) and ApoB-100-derived antigenic peptide P210 are engineered. Polymersomes decorated with an optimized surface display and density for a lipid construct of the P-D2 peptide, which binds CD11c on the DC surface, significantly enhance the cytosolic delivery and resulting immunomodulatory capacity of aVD in vitro. Weekly low-dose intravenous administration of DC-targeted, aVD-loaded polymersomes significantly inhibit atherosclerotic lesion development in high-fat-diet-fed ApoE−/− mice. The results validate the key role of DC immunomodulation during aVD-dependent inhibition of atherosclerosis and demonstrate the therapeutic enhancement and dosage lowering capability of cell-targeted nanotherapy in the treatment of CVD.

Original languageEnglish
Article number1904399
JournalAdvanced Functional Materials
DOIs
Publication statusAccepted/In press - Jan 1 2019

Keywords

  • atherosclerosis
  • dendritic cells
  • immunotherapy
  • polymersomes
  • targeted delivery

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Electrochemistry

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  • Cite this

    Yi, S., Zhang, X., Sangji, M. H., Liu, Y., Allen, S. D., Xiao, B., Bobbala, S., Braverman, C. L., Cai, L., Hecker, P. I., DeBerge, M., Thorp, E. B., Temel, R. E., Stupp, S. I., & Scott, E. A. (Accepted/In press). Surface Engineered Polymersomes for Enhanced Modulation of Dendritic Cells During Cardiovascular Immunotherapy. Advanced Functional Materials, [1904399]. https://doi.org/10.1002/adfm.201904399