Targeted Nitric Oxide Delivery by Supramolecular Nanofibers for the Prevention of Restenosis after Arterial Injury

Edward S M Bahnson, Hussein A. Kassam, Tyson J. Moyer, Wulin Jiang, Courtney E. Morgan, Janet M. Vercammen, Qun Jiang, Megan E. Flynn, Samuel I Stupp, Melina R. Kibbe

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Cardiovascular interventions continue to fail as a result of arterial restenosis secondary to neointimal hyperplasia. We sought to develop and evaluate a systemically delivered nanostructure targeted to the site of arterial injury to prevent neointimal hyperplasia. Nanostructures were based on self-assembling biodegradable molecules known as peptide amphiphiles. The targeting motif was a collagen-binding peptide, and the therapeutic moiety was added by S-nitrosylation of cysteine residues. Results: Structure of the nanofibers was characterized by transmission electron microscopy and small-angle X-ray scattering. S-nitrosylation was confirmed by mass spectrometry, and nitric oxide (NO) release was assessed electrochemically and by chemiluminescent detection. The balloon carotid artery injury model was performed on 10-week-old male Sprague-Dawley rats. Immediately after injury, nanofibers were administered systemically via tail vein injection. S-nitrosylated (S-nitrosyl [SNO])-targeted nanofibers significantly reduced neointimal hyperplasia 2 weeks and 7 months following balloon angioplasty, with no change in inflammation. Innovation: This is the first time that an S-nitrosothiol (RSNO)-based therapeutic was shown to have targeted local effects after systemic administration. This approach, combining supramolecular nanostructures with a therapeutic NO-based payload and a targeting moiety, overcomes the limitations of delivering NO to a site of interest, avoiding undesirable systemic side effects. Conclusion: We successfully synthesized and characterized an RSNO-based therapy that when administered systemically, targets directly to the site of vascular injury. By integrating therapeutic and targeting chemistries, these targeted SNO nanofibers provided durable inhibition of neointimal hyperplasia in vivo and show great potential as a platform to treat cardiovascular diseases.

Original languageEnglish
Pages (from-to)401-418
Number of pages18
JournalAntioxidants and Redox Signaling
Volume24
Issue number8
DOIs
Publication statusPublished - Mar 10 2016

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Nanofibers
Nitric Oxide
Hyperplasia
Nanostructures
Balloons
Wounds and Injuries
S-Nitrosothiols
Peptides
Amphiphiles
Carotid Artery Injuries
Therapeutics
X ray scattering
Balloon Angioplasty
Vascular System Injuries
Mass spectrometry
Cysteine
Rats
Transmission Electron Microscopy
Collagen
Innovation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Physiology
  • Clinical Biochemistry

Cite this

Bahnson, E. S. M., Kassam, H. A., Moyer, T. J., Jiang, W., Morgan, C. E., Vercammen, J. M., ... Kibbe, M. R. (2016). Targeted Nitric Oxide Delivery by Supramolecular Nanofibers for the Prevention of Restenosis after Arterial Injury. Antioxidants and Redox Signaling, 24(8), 401-418. https://doi.org/10.1089/ars.2015.6363

Targeted Nitric Oxide Delivery by Supramolecular Nanofibers for the Prevention of Restenosis after Arterial Injury. / Bahnson, Edward S M; Kassam, Hussein A.; Moyer, Tyson J.; Jiang, Wulin; Morgan, Courtney E.; Vercammen, Janet M.; Jiang, Qun; Flynn, Megan E.; Stupp, Samuel I; Kibbe, Melina R.

In: Antioxidants and Redox Signaling, Vol. 24, No. 8, 10.03.2016, p. 401-418.

Research output: Contribution to journalArticle

Bahnson, ESM, Kassam, HA, Moyer, TJ, Jiang, W, Morgan, CE, Vercammen, JM, Jiang, Q, Flynn, ME, Stupp, SI & Kibbe, MR 2016, 'Targeted Nitric Oxide Delivery by Supramolecular Nanofibers for the Prevention of Restenosis after Arterial Injury', Antioxidants and Redox Signaling, vol. 24, no. 8, pp. 401-418. https://doi.org/10.1089/ars.2015.6363
Bahnson, Edward S M ; Kassam, Hussein A. ; Moyer, Tyson J. ; Jiang, Wulin ; Morgan, Courtney E. ; Vercammen, Janet M. ; Jiang, Qun ; Flynn, Megan E. ; Stupp, Samuel I ; Kibbe, Melina R. / Targeted Nitric Oxide Delivery by Supramolecular Nanofibers for the Prevention of Restenosis after Arterial Injury. In: Antioxidants and Redox Signaling. 2016 ; Vol. 24, No. 8. pp. 401-418.
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