Template-directed synthesis of self-assembled inorganic nanostructures

Linda A. Stearns, Rahul Chhabra, William T. Petuskey, Hao Yan, John C. Chaput

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The A3 peptide, discovered by in vitro evolution, has been shown to bind silver and gold nanoparticles. We chemically conjugated the A3 peptide to a 20-mer oligonucleotide, thereby generating an A3 peptide-oligonucleotide conjugate (A3 POC). The A3 POC bears the complementary sequence to 10 single-stranded DNA "capture" probes extending outward from the surface of an origami DNA fixed-length nanotube. After assembling the A3 POC-nanotube complex in Hepes buffer, AuCl4-was added to induce Au nanoparticle (NP) nucleation and growth. Transmission electron microscopy revealed that a majority of the fixed-length tubes were decorated with 5 to 6 Au NPs (~10 nm in diameter) each, while a negative control (no A3 POC) showed NPs that were not localized to the DNA tubes. At present, we are working to optimize Au NP nucleation to achieve the maximum of 10 NPs per tube, while minimizing salt-induced aggregation effects.

Original languageEnglish
Title of host publicationExtended Abstracts for 6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009
Pages89-92
Number of pages4
Publication statusPublished - 2009
Event6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009 - Snowbird, UT, United States
Duration: Apr 20 2009Apr 24 2009

Other

Other6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009
CountryUnited States
CitySnowbird, UT
Period4/20/094/24/09

Fingerprint

Oligonucleotides
Peptides
Nanostructures
DNA
Nanoparticles
Nanotubes
Nucleation
Silver
Agglomeration
Gold
Salts
Transmission electron microscopy

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Stearns, L. A., Chhabra, R., Petuskey, W. T., Yan, H., & Chaput, J. C. (2009). Template-directed synthesis of self-assembled inorganic nanostructures. In Extended Abstracts for 6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009 (pp. 89-92)

Template-directed synthesis of self-assembled inorganic nanostructures. / Stearns, Linda A.; Chhabra, Rahul; Petuskey, William T.; Yan, Hao; Chaput, John C.

Extended Abstracts for 6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009. 2009. p. 89-92.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Stearns, LA, Chhabra, R, Petuskey, WT, Yan, H & Chaput, JC 2009, Template-directed synthesis of self-assembled inorganic nanostructures. in Extended Abstracts for 6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009. pp. 89-92, 6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009, Snowbird, UT, United States, 4/20/09.
Stearns LA, Chhabra R, Petuskey WT, Yan H, Chaput JC. Template-directed synthesis of self-assembled inorganic nanostructures. In Extended Abstracts for 6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009. 2009. p. 89-92
Stearns, Linda A. ; Chhabra, Rahul ; Petuskey, William T. ; Yan, Hao ; Chaput, John C. / Template-directed synthesis of self-assembled inorganic nanostructures. Extended Abstracts for 6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009. 2009. pp. 89-92
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