Abstract
We have investigated the effects of temperature on the formation and decay of the light-induced multiline EPR signal species associated with photosynthetic oxygen evolution (Dismukes, G.C. and Siderer, Y. (1980) FEBS Lett. 121, 78-80). (1) The decay rate following illumination is temperature dependent: at 295 K the half-time of decay is about 40 s, at 253 K the half-time is approx. 40 min. (2) A single intense flash of light becomes progressively less effective in generating the multiline signal below about 240 K. (3) Continuous illumination is capable of generating the signal down to almost 160 K. (4) Continuous illumination after a preilluminating flash generates less signal above 200 K than at lower temperatures. Our results support the conclusion of Dismukes and Siderer that the S2 state gives rise to this multiline signal; we find that the S1 state can be fully advanced to the S2 state at temperatures as low as 160 K. The S2 state is capable of further advancement at temperatures above about 210 K, but not below that temperature.
| Original language | English |
|---|---|
| Pages (from-to) | 366-371 |
| Number of pages | 6 |
| Journal | Biochimica et Biophysica Acta - Bioenergetics |
| Volume | 723 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 30 1983 |
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Keywords
- (Spinach chloroplast)
- ESR
- Oxygen evolution
- Photosynthesis
- Temperature effect
ASJC Scopus subject areas
- Biophysics
Cite this
The effect of temperature on the formation and decay of the multiline EPR signal species associated with photosynthetic oxygen evolution. / Brudvig, Gary W; Casey, John L.; Sauer, Kenneth.
In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 723, No. 3, 30.06.1983, p. 366-371.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of temperature on the formation and decay of the multiline EPR signal species associated with photosynthetic oxygen evolution
AU - Brudvig, Gary W
AU - Casey, John L.
AU - Sauer, Kenneth
PY - 1983/6/30
Y1 - 1983/6/30
N2 - We have investigated the effects of temperature on the formation and decay of the light-induced multiline EPR signal species associated with photosynthetic oxygen evolution (Dismukes, G.C. and Siderer, Y. (1980) FEBS Lett. 121, 78-80). (1) The decay rate following illumination is temperature dependent: at 295 K the half-time of decay is about 40 s, at 253 K the half-time is approx. 40 min. (2) A single intense flash of light becomes progressively less effective in generating the multiline signal below about 240 K. (3) Continuous illumination is capable of generating the signal down to almost 160 K. (4) Continuous illumination after a preilluminating flash generates less signal above 200 K than at lower temperatures. Our results support the conclusion of Dismukes and Siderer that the S2 state gives rise to this multiline signal; we find that the S1 state can be fully advanced to the S2 state at temperatures as low as 160 K. The S2 state is capable of further advancement at temperatures above about 210 K, but not below that temperature.
AB - We have investigated the effects of temperature on the formation and decay of the light-induced multiline EPR signal species associated with photosynthetic oxygen evolution (Dismukes, G.C. and Siderer, Y. (1980) FEBS Lett. 121, 78-80). (1) The decay rate following illumination is temperature dependent: at 295 K the half-time of decay is about 40 s, at 253 K the half-time is approx. 40 min. (2) A single intense flash of light becomes progressively less effective in generating the multiline signal below about 240 K. (3) Continuous illumination is capable of generating the signal down to almost 160 K. (4) Continuous illumination after a preilluminating flash generates less signal above 200 K than at lower temperatures. Our results support the conclusion of Dismukes and Siderer that the S2 state gives rise to this multiline signal; we find that the S1 state can be fully advanced to the S2 state at temperatures as low as 160 K. The S2 state is capable of further advancement at temperatures above about 210 K, but not below that temperature.
KW - (Spinach chloroplast)
KW - ESR
KW - Oxygen evolution
KW - Photosynthesis
KW - Temperature effect
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UR - http://www.scopus.com/inward/citedby.url?scp=0000269978&partnerID=8YFLogxK
U2 - 10.1016/0005-2728(83)90042-7
DO - 10.1016/0005-2728(83)90042-7
M3 - Article
AN - SCOPUS:0000269978
VL - 723
SP - 366
EP - 371
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
SN - 0005-2728
IS - 3
ER -