The interaction of spinach nitrite reductase with ferredoxin

A site-directed mutation study

Masakazu Hirasawa, Jatindra N. Tripathy, Ramasamy Somasundaram, Michael K. Johnson, Megha Bhalla, James Paul Allen, David B. Knaff

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A series of site-directed mutants of the ferredoxin-dependent spinach nitrite reductase has been characterized and several amino acids have been identified that appear to be involved in the interaction of the enzyme with ferredoxin. In a complementary study, binding constants to nitrite reductase and steady-state kinetic parameters of site-directed mutants of ferredoxin were determined in an attempt to identify ferredoxin amino acids involved in the interaction with nitrite reductase. The results have been interpreted in terms of an in-silico docking model for the 1:1 complex of ferredoxin with nitrite reductase.

Original languageEnglish
Pages (from-to)407-415
Number of pages9
JournalMolecular Plant
Volume2
Issue number3
DOIs
Publication statusPublished - May 2009

Fingerprint

Ferredoxin-Nitrite Reductase
Ferredoxins
nitrite reductase
ferredoxins
Spinacia oleracea
Nitrite Reductases
spinach
mutation
Mutation
Amino Acids
mutants
amino acids
Computer Simulation
Enzymes
kinetics
enzymes

Keywords

  • Electron transport
  • Enzymology
  • Molecular biology
  • Nitrogen metabolism

ASJC Scopus subject areas

  • Plant Science
  • Molecular Biology

Cite this

Hirasawa, M., Tripathy, J. N., Somasundaram, R., Johnson, M. K., Bhalla, M., Allen, J. P., & Knaff, D. B. (2009). The interaction of spinach nitrite reductase with ferredoxin: A site-directed mutation study. Molecular Plant, 2(3), 407-415. https://doi.org/10.1093/mp/ssn098

The interaction of spinach nitrite reductase with ferredoxin : A site-directed mutation study. / Hirasawa, Masakazu; Tripathy, Jatindra N.; Somasundaram, Ramasamy; Johnson, Michael K.; Bhalla, Megha; Allen, James Paul; Knaff, David B.

In: Molecular Plant, Vol. 2, No. 3, 05.2009, p. 407-415.

Research output: Contribution to journalArticle

Hirasawa, M, Tripathy, JN, Somasundaram, R, Johnson, MK, Bhalla, M, Allen, JP & Knaff, DB 2009, 'The interaction of spinach nitrite reductase with ferredoxin: A site-directed mutation study', Molecular Plant, vol. 2, no. 3, pp. 407-415. https://doi.org/10.1093/mp/ssn098
Hirasawa, Masakazu ; Tripathy, Jatindra N. ; Somasundaram, Ramasamy ; Johnson, Michael K. ; Bhalla, Megha ; Allen, James Paul ; Knaff, David B. / The interaction of spinach nitrite reductase with ferredoxin : A site-directed mutation study. In: Molecular Plant. 2009 ; Vol. 2, No. 3. pp. 407-415.
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