The internal structure of self-assembled peptide amphiphiles nanofibers

Hongzhou Jiang, Mustafa O. Guler, Samuel I. Stupp

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The self-assembly of peptide amphiphiles (PAs) into nanofibers and their bioactivity as well as physical properties have been investigated by our laboratory over the past few years. We report here on the use of transmission infrared spectroscopy (IR) and polarization modulation-infrared reflection-absorption spectroscopy (PM-IRRAS) to characterize the internal structure of the nanofibers. Depositing nanofibers flat on surfaces, and using the surface selection rules in PM-IRRAS, we demonstrate that peptide amphiphiles form β-sheets oriented parallel to the long-axis of nanofibers that pack radially from the nanofiber core. We show also that the extent of internal order depends on the molecular architecture and peptide sequence of PAs, with branched PAs yielding nanofibers with the lowest degree of internal order. Measurements of intensity and spectral position of the alkyl bands suggest that the hydrophobic core of these nanofibers can have internal order to an extent that correlates with order in their peptidic domains. We expect that bioactivity and physical properties will be controlled by the degree of internal order in these self-assembling nanostructures.

Original languageEnglish
Pages (from-to)454-462
Number of pages9
JournalSoft Matter
Volume3
Issue number4
DOIs
Publication statusPublished - Mar 27 2007

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

Fingerprint Dive into the research topics of 'The internal structure of self-assembled peptide amphiphiles nanofibers'. Together they form a unique fingerprint.

  • Cite this