The quinone acceptor A 1 in photosystem I: Binding site, and comparison to Q A in purple bacteria reaction centers

Andreas Kamlowski, Brigitte Altenberg-Greulich, Arthur Van Der Est, Stephan G. Zech, Robert Bittl, Petra Fromme, Wolfgang Lubitz, Dietmar Stehlik

Research output: Contribution to journalArticle

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Abstract

The nature of the binding site of the quinone acceptor A 1 in Photosystem I (PSI) is studied by modeling the protein and cofactor on the basis of structural data derived from the intermediate resolution 4 Å X-ray diffraction electron density map, the position and orientation of A 1 as evaluated from EPR data, and the histidine ligation of P 700 as deduced from mutation experiments. Several models are constructed within the degrees of freedom allowed by the experimental constraints. In all cases a close interaction between the A 1 headgroup and the side chain of PsaA-Trp697 (PsaB-Trp677) is found. The model is compared to the known binding site of Q A in bacterial reaction centers (bRC) in which a similar quinone-tryptophan arrangement has been established. The results are also compared for consistency with published magnetic resonance data. The influences of the protein environment on the semiquinone g-tensor and hyperfine couplings are considerably different in PSI and bRC. It is argued that this is mainly a result of differences in the hydrogen bonding to the protein, in the strength of the π-π interactions with the tryptophan, and in the protein induced asymmetry in the spin density of the respective quinone radical anion.

Original languageEnglish
Pages (from-to)8278-8287
Number of pages10
JournalJournal of Physical Chemistry B
Volume102
Issue number42
Publication statusPublished - Oct 15 1998

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Photosystem I Protein Complex
quinones
Binding sites
bacteria
Binding Sites
proteins
Proteins
tryptophan
Tryptophan
histidine
Magnetic resonance
mutations
Histidine
Tensors
Anions
Carrier concentration
Paramagnetic resonance
magnetic resonance
Hydrogen bonds
Negative ions

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Kamlowski, A., Altenberg-Greulich, B., Van Der Est, A., Zech, S. G., Bittl, R., Fromme, P., ... Stehlik, D. (1998). The quinone acceptor A 1 in photosystem I: Binding site, and comparison to Q A in purple bacteria reaction centers. Journal of Physical Chemistry B, 102(42), 8278-8287.

The quinone acceptor A 1 in photosystem I : Binding site, and comparison to Q A in purple bacteria reaction centers. / Kamlowski, Andreas; Altenberg-Greulich, Brigitte; Van Der Est, Arthur; Zech, Stephan G.; Bittl, Robert; Fromme, Petra; Lubitz, Wolfgang; Stehlik, Dietmar.

In: Journal of Physical Chemistry B, Vol. 102, No. 42, 15.10.1998, p. 8278-8287.

Research output: Contribution to journalArticle

Kamlowski, A, Altenberg-Greulich, B, Van Der Est, A, Zech, SG, Bittl, R, Fromme, P, Lubitz, W & Stehlik, D 1998, 'The quinone acceptor A 1 in photosystem I: Binding site, and comparison to Q A in purple bacteria reaction centers', Journal of Physical Chemistry B, vol. 102, no. 42, pp. 8278-8287.
Kamlowski A, Altenberg-Greulich B, Van Der Est A, Zech SG, Bittl R, Fromme P et al. The quinone acceptor A 1 in photosystem I: Binding site, and comparison to Q A in purple bacteria reaction centers. Journal of Physical Chemistry B. 1998 Oct 15;102(42):8278-8287.
Kamlowski, Andreas ; Altenberg-Greulich, Brigitte ; Van Der Est, Arthur ; Zech, Stephan G. ; Bittl, Robert ; Fromme, Petra ; Lubitz, Wolfgang ; Stehlik, Dietmar. / The quinone acceptor A 1 in photosystem I : Binding site, and comparison to Q A in purple bacteria reaction centers. In: Journal of Physical Chemistry B. 1998 ; Vol. 102, No. 42. pp. 8278-8287.
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