The radical pair state P .+ 700A .- 1 in photosystem I single crystals

Orientation dependence of the transient spin-polarized EPR spectra

Andreas Kamlowski, Stephan G. Zech, Petra Fromme, Robert Bittl, Wolfgang Lubitz, Horst T. Witt, Dietmar Stehlik

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The light-induced, charge-separated state P .+ 700A .- 1 in single crystals of Photosystem I (PSI) from the cyanobacterium Synechococcus elongatus is investigated with transient, direct-detection EPR spectroscopy. The orientation of the phylloquinone head group of A 1 within the PSI reaction center is determined from the orientation dependence of the spin-polarized X-band EPR spectrum of the radical pair P .+ 700A .- 1 made up of the primary donor, P 700, and the acceptor, A 1. From the angular dependence of the overall spin-polarization pattern an upper limit 〈(c, z d) ≤ 30° is evaluated for the angle between the crystallographic c-axis, collinear with the membrane normal, and the dipolar axis, z d, connecting the electron spin density centers of P .+ 700 and A .- 1. A partially resolved hyperfine coupling (hfc) is assigned to the hfc tensor of the 2-methyl group of A 1. Its A a principal axis encloses an angle of β = 35°-55° with c. Simulations of the rotation patterns support a lower limit for the angle 〈(c, z d) ≥ 25° with a larger error than for the upper limit. z d is confirmed to be parallel to both the g xx principal axis of g(A .- 1) and to the C=O carbonyl bonds within ±5°. With respect to rotation around the g xx axis, the angle between the (c, z d) plane and the quinone plane of the A .- 1 head group can only be specified within an upper limit of 60°. Together with independent knowledge about the location of A 1 within the PSI reaction center, a nearly complete structural model for the head group of the functional A 1 cofactor is achieved.

Original languageEnglish
Pages (from-to)8266-8277
Number of pages12
JournalJournal of Physical Chemistry B
Volume102
Issue number42
Publication statusPublished - Oct 15 1998

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Photosystem I Protein Complex
Crystal orientation
Paramagnetic resonance
Single crystals
Spin polarization
single crystals
Vitamin K 1
Tensors
phylloquinone
Spectroscopy
Membranes
Electrons
quinones
superhigh frequencies
electron spin
tensors
membranes
polarization
spectroscopy
simulation

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

The radical pair state P .+ 700A .- 1 in photosystem I single crystals : Orientation dependence of the transient spin-polarized EPR spectra. / Kamlowski, Andreas; Zech, Stephan G.; Fromme, Petra; Bittl, Robert; Lubitz, Wolfgang; Witt, Horst T.; Stehlik, Dietmar.

In: Journal of Physical Chemistry B, Vol. 102, No. 42, 15.10.1998, p. 8266-8277.

Research output: Contribution to journalArticle

Kamlowski, Andreas ; Zech, Stephan G. ; Fromme, Petra ; Bittl, Robert ; Lubitz, Wolfgang ; Witt, Horst T. ; Stehlik, Dietmar. / The radical pair state P .+ 700A .- 1 in photosystem I single crystals : Orientation dependence of the transient spin-polarized EPR spectra. In: Journal of Physical Chemistry B. 1998 ; Vol. 102, No. 42. pp. 8266-8277.
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