Abstract
We have investigated the importance of carotenoids on the accumulation and function of the photosynthetic apparatus using a mutant of the green alga Chlamydomonas reinhardtii lacking carotenoids. The FN68 mutant is deficient in phytoene synthase, the first enzyme of the carotenoid biosynthesis pathway, and therefore is unable to synthesize any carotenes and xanthophylls. We find that FN68 is unable to accumulate the light-harvesting complexes associated with both photosystems as well as the RC subunits of photosystem II. The accumulation of the cytochrome b6f complex is also strongly reduced to a level approximately 10% that of the wild type. However, the residual fraction of assembled cytochrome b6f complexes exhibits single-turnover electron transfer kinetics comparable to those observed in the wild-type strain. Surprisingly, photosystem I is assembled to significant levels in the absence of carotenoids in FN68 and possesses functional properties that are very similar to those of the wild-type complex.
| Original language | English |
|---|---|
| Pages (from-to) | 535-546 |
| Number of pages | 12 |
| Journal | Plant Physiology |
| Volume | 161 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2013 |
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ASJC Scopus subject areas
- Plant Science
- Genetics
- Physiology
Cite this
The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii. / Santabarbara, Stefano; Casazza, Anna Paola; Ali, Kulsam; Economou, Chloe K.; Wannathong, Thanyanun; Zito, Francesca; Redding, Kevin Edward; Rappaport, Fabrice; Purton, Saul.
In: Plant Physiology, Vol. 161, No. 1, 01.2013, p. 535-546.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii
AU - Santabarbara, Stefano
AU - Casazza, Anna Paola
AU - Ali, Kulsam
AU - Economou, Chloe K.
AU - Wannathong, Thanyanun
AU - Zito, Francesca
AU - Redding, Kevin Edward
AU - Rappaport, Fabrice
AU - Purton, Saul
PY - 2013/1
Y1 - 2013/1
N2 - We have investigated the importance of carotenoids on the accumulation and function of the photosynthetic apparatus using a mutant of the green alga Chlamydomonas reinhardtii lacking carotenoids. The FN68 mutant is deficient in phytoene synthase, the first enzyme of the carotenoid biosynthesis pathway, and therefore is unable to synthesize any carotenes and xanthophylls. We find that FN68 is unable to accumulate the light-harvesting complexes associated with both photosystems as well as the RC subunits of photosystem II. The accumulation of the cytochrome b6f complex is also strongly reduced to a level approximately 10% that of the wild type. However, the residual fraction of assembled cytochrome b6f complexes exhibits single-turnover electron transfer kinetics comparable to those observed in the wild-type strain. Surprisingly, photosystem I is assembled to significant levels in the absence of carotenoids in FN68 and possesses functional properties that are very similar to those of the wild-type complex.
AB - We have investigated the importance of carotenoids on the accumulation and function of the photosynthetic apparatus using a mutant of the green alga Chlamydomonas reinhardtii lacking carotenoids. The FN68 mutant is deficient in phytoene synthase, the first enzyme of the carotenoid biosynthesis pathway, and therefore is unable to synthesize any carotenes and xanthophylls. We find that FN68 is unable to accumulate the light-harvesting complexes associated with both photosystems as well as the RC subunits of photosystem II. The accumulation of the cytochrome b6f complex is also strongly reduced to a level approximately 10% that of the wild type. However, the residual fraction of assembled cytochrome b6f complexes exhibits single-turnover electron transfer kinetics comparable to those observed in the wild-type strain. Surprisingly, photosystem I is assembled to significant levels in the absence of carotenoids in FN68 and possesses functional properties that are very similar to those of the wild-type complex.
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U2 - 10.1104/pp.112.205260
DO - 10.1104/pp.112.205260
M3 - Article
C2 - 23161889
AN - SCOPUS:84871811006
VL - 161
SP - 535
EP - 546
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 1
ER -