The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii

Stefano Santabarbara, Anna Paola Casazza, Kulsam Ali, Chloe K. Economou, Thanyanun Wannathong, Francesca Zito, Kevin Edward Redding, Fabrice Rappaport, Saul Purton

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have investigated the importance of carotenoids on the accumulation and function of the photosynthetic apparatus using a mutant of the green alga Chlamydomonas reinhardtii lacking carotenoids. The FN68 mutant is deficient in phytoene synthase, the first enzyme of the carotenoid biosynthesis pathway, and therefore is unable to synthesize any carotenes and xanthophylls. We find that FN68 is unable to accumulate the light-harvesting complexes associated with both photosystems as well as the RC subunits of photosystem II. The accumulation of the cytochrome b6f complex is also strongly reduced to a level approximately 10% that of the wild type. However, the residual fraction of assembled cytochrome b6f complexes exhibits single-turnover electron transfer kinetics comparable to those observed in the wild-type strain. Surprisingly, photosystem I is assembled to significant levels in the absence of carotenoids in FN68 and possesses functional properties that are very similar to those of the wild-type complex.

Original languageEnglish
Pages (from-to)535-546
Number of pages12
JournalPlant Physiology
Volume161
Issue number1
DOIs
Publication statusPublished - Jan 2013

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Photosynthetic Reaction Center Complex Proteins
Chlamydomonas reinhardtii
Carotenoids
carotenoids
Cytochrome b6f Complex
cytochromes
phytoene synthase
mutants
xanthophylls
light harvesting complex
Xanthophylls
photosystem I
Photosystem I Protein Complex
carotenes
Chlorophyta
Photosystem II Protein Complex
photosystem II
electron transfer
functional properties
biosynthesis

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Santabarbara, S., Casazza, A. P., Ali, K., Economou, C. K., Wannathong, T., Zito, F., ... Purton, S. (2013). The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii. Plant Physiology, 161(1), 535-546. https://doi.org/10.1104/pp.112.205260

The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii. / Santabarbara, Stefano; Casazza, Anna Paola; Ali, Kulsam; Economou, Chloe K.; Wannathong, Thanyanun; Zito, Francesca; Redding, Kevin Edward; Rappaport, Fabrice; Purton, Saul.

In: Plant Physiology, Vol. 161, No. 1, 01.2013, p. 535-546.

Research output: Contribution to journalArticle

Santabarbara, S, Casazza, AP, Ali, K, Economou, CK, Wannathong, T, Zito, F, Redding, KE, Rappaport, F & Purton, S 2013, 'The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii', Plant Physiology, vol. 161, no. 1, pp. 535-546. https://doi.org/10.1104/pp.112.205260
Santabarbara, Stefano ; Casazza, Anna Paola ; Ali, Kulsam ; Economou, Chloe K. ; Wannathong, Thanyanun ; Zito, Francesca ; Redding, Kevin Edward ; Rappaport, Fabrice ; Purton, Saul. / The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii. In: Plant Physiology. 2013 ; Vol. 161, No. 1. pp. 535-546.
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