The role of tryptophan in the ferredoxin-dependent nitrite reductase of spinach

Jatindra N. Tripathy, Masakazu Hirasawa, Sung Kun Kim, Aaron T. Setterdahl, James Paul Allen, David B. Knaff

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A system has been developed for expressing a His-tagged form of the ferredoxin-dependent nitrite reductase of spinach in Escherichia coli. The catalytic and spectral properties of the His-tagged, recombinant enzyme are similar, but not identical, to those previously observed for nitrite reductase isolated directly from spinach leaf. A detailed comparison of the spectral, catalytic and fluorescence properties of nitrite reductase variants, in which each of the enzyme's eight tryptophan residues has been replaced using site-directed mutagenesis by either aromatic or non-aromatic amino acids, has been used to examine possible roles for tryptophan residues in the reduction of nitrite to ammonia catalyzed by the enzyme.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalPhotosynthesis Research
Volume94
Issue number1
DOIs
Publication statusPublished - Oct 2007

Fingerprint

Ferredoxin-Nitrite Reductase
Nitrite Reductases
Ferredoxins
nitrite reductase
ferredoxins
Spinacia oleracea
spinach
Tryptophan
tryptophan
Enzymes
enzymes
Mutagenesis
site-directed mutagenesis
Nitrites
Site-Directed Mutagenesis
Ammonia
nitrites
Escherichia coli
aromatic compounds
ammonia

Keywords

  • Ferredoxin
  • Nitrite reductase
  • Tryptophan

ASJC Scopus subject areas

  • Plant Science

Cite this

The role of tryptophan in the ferredoxin-dependent nitrite reductase of spinach. / Tripathy, Jatindra N.; Hirasawa, Masakazu; Kim, Sung Kun; Setterdahl, Aaron T.; Allen, James Paul; Knaff, David B.

In: Photosynthesis Research, Vol. 94, No. 1, 10.2007, p. 1-12.

Research output: Contribution to journalArticle

Tripathy, Jatindra N. ; Hirasawa, Masakazu ; Kim, Sung Kun ; Setterdahl, Aaron T. ; Allen, James Paul ; Knaff, David B. / The role of tryptophan in the ferredoxin-dependent nitrite reductase of spinach. In: Photosynthesis Research. 2007 ; Vol. 94, No. 1. pp. 1-12.
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