The structure of the heterodimer reaction center from Rhodobacter sphaeroides at 2.55 Å resolution

A. Camara-Artigas, C. Magee, A. Goetsch, James Paul Allen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Crystals have been obtained of reaction centers of the heterodimer mutant that has significantly different properties than wild type due to the primary donor being formed from both a bacteriochlorophyll and bacteriopheophytin rather than two bacteriochlorophylls as found for wild type. The crystals belong to the trigonal space group P3121 and the structure has been refined to a resolution limit of 2.55 Å with an R factor of 19.0%. The electron density maps confirm that a primary donor does indeed contain a bacteriopheophytin due to the His to Leu substitution at M202 that coordinates the corresponding bacteriochlorophyll in wild-type. Other structural changes compared to wild type are relatively minor with the relative orientation and positioning of the two tetrapyrroles forming the primary donor being unchanged within the error. Compared to wild type, the only significant alterations are small shifts of residues M196 to M206, a rotation of the side chain of Ile M206, and the loss of a bound water molecule that in wild-type is hydrogen-bonded to both His M202 and the bacteriochlorophyll monomer on the active branch. Since hydrogen-bonding interactions strongly influence the energies of tetrapyrroles, the loss of the water molecule should result in changes in the energies of the bacteriochlorophyll monomer that contributes to the observed functional differences with wild-type.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalPhotosynthesis Research
Volume74
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Bacteriochlorophylls
Rhodobacter sphaeroides
crystals
bound water
hydrogen bonding
Tetrapyrroles
energy
hydrogen
electrons
mutants
Monomers
Crystals
Molecules
Water
R Factors
Hydrogen Bonding
water
Carrier concentration
Hydrogen
Hydrogen bonds

Keywords

  • Bacteriochlorophyll
  • Electron donor
  • Muragenesis
  • Purple bacteria
  • X-ray diffraction

ASJC Scopus subject areas

  • Plant Science

Cite this

The structure of the heterodimer reaction center from Rhodobacter sphaeroides at 2.55 Å resolution. / Camara-Artigas, A.; Magee, C.; Goetsch, A.; Allen, James Paul.

In: Photosynthesis Research, Vol. 74, No. 1, 2002, p. 87-93.

Research output: Contribution to journalArticle

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