The three-dimensional structures of bacterial reaction centers

T. L. Olson, J. C. Williams, James Paul Allen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This review presents a broad overview of the research that enabled the structure determination of the bacterial reaction centers from Blastochloris viridis and Rhodobacter sphaeroides, with a focus on the contributions from Duysens, Clayton, and Feher. Early experiments performed in the laboratory of Duysens and others demonstrated the utility of spectroscopic techniques and the presence of photosynthetic complexes in both oxygenic and anoxygenic photosynthesis. The laboratories of Clayton and Feher led efforts to isolate and characterize the bacterial reaction centers. The availability of well-characterized preparations of pure and stable reaction centers allowed the crystallization and subsequent determination of the structures using X-ray diffraction. The three-dimensional structures of reaction centers revealed an overall arrangement of two symmetrical branches of cofactors surrounded by transmembrane helices from the L and M subunits, which also are related by the same twofold symmetry axis. The structure has served as a framework to address several issues concerning bacterial photosynthesis, including the directionality of electron transfer, the properties of the reaction center-cytochrome c 2 complex, and the coupling of proton and electron transfer. Together, these research efforts laid the foundation for ongoing efforts to address an outstanding question in oxygenic photosynthesis, namely the molecular mechanism of water oxidation.

Original languageEnglish
Pages (from-to)87-98
Number of pages12
JournalPhotosynthesis Research
Volume120
Issue number1-2
DOIs
Publication statusPublished - 2014

Fingerprint

Bacterial Structures
Photosynthesis
photosynthesis
electron transfer
Blastochloris viridis
Electrons
Photosynthetic Reaction Center Complex Proteins
Rhodobacter sphaeroides
cytochrome c
crystallization
Crystallization
Cytochromes c
Research
X-ray diffraction
X-Ray Diffraction
protons
Protons
Availability
oxidation
X ray diffraction

Keywords

  • Blastochloris viridis
  • Membrane protein
  • Protein purification
  • Rhodobacter sphaeroides
  • Spectroscopy
  • X-ray diffraction

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Biochemistry
  • Medicine(all)

Cite this

The three-dimensional structures of bacterial reaction centers. / Olson, T. L.; Williams, J. C.; Allen, James Paul.

In: Photosynthesis Research, Vol. 120, No. 1-2, 2014, p. 87-98.

Research output: Contribution to journalArticle

Olson, T. L. ; Williams, J. C. ; Allen, James Paul. / The three-dimensional structures of bacterial reaction centers. In: Photosynthesis Research. 2014 ; Vol. 120, No. 1-2. pp. 87-98.
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