Thermal response of DNA supramolecular polymers assembled with hydrophobic sticky ends

Mahesh Hariharan, Yan Zheng, Boris Rybtchinski, Frederick D. Lewis

Research output: Contribution to journalArticle

Abstract

We report the self-assembly and thermal dissociation of DNA dumbbell conjugates having a perylenediimide (PDI) linker on each end separated by 6-16 A-T base pairs. In the presence of NaCl these dumbbells form one-dimensional supramolecular assemblies as a consequence of the hydrophobic association of their PDI sticky ends. The dependence of assembly formation on dumbbell concentration, salt concentration, and temperature can be conveniently monitored by UV-vis spectroscopy. The melting of these linear assemblies follows two limiting mechanisms, depending on the length of the dumbbells. Upon heating in the presence of salt, the assemblies formed by the longer dumbbells undergo a sequential transition from assembly to base-paired monomer to random coiled monomer, whereas the assemblies formed by the shorter dumbbells undergo disassembly and base-pair melting cooperatively. In all cases, the intramolecular hydrophobic association of the PDI chromophores is observed at elevated temperature. The thermal behavior of these one-dimensional assemblies is compared to that of other sticky-ended assemblies.

Original languageEnglish
Pages (from-to)14649-14654
Number of pages6
JournalJournal of Physical Chemistry B
Volume117
Issue number47
DOIs
Publication statusPublished - Nov 27 2013

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assemblies
Polymers
DNA
deoxyribonucleic acid
polymers
monomers
assembly
melting
salts
thermal dissociation
Melting
Salts
Monomers
Association reactions
chromophores
Hot Temperature
self assembly
Chromophores
Ultraviolet spectroscopy
dissociation

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

Thermal response of DNA supramolecular polymers assembled with hydrophobic sticky ends. / Hariharan, Mahesh; Zheng, Yan; Rybtchinski, Boris; Lewis, Frederick D.

In: Journal of Physical Chemistry B, Vol. 117, No. 47, 27.11.2013, p. 14649-14654.

Research output: Contribution to journalArticle

Hariharan, Mahesh ; Zheng, Yan ; Rybtchinski, Boris ; Lewis, Frederick D. / Thermal response of DNA supramolecular polymers assembled with hydrophobic sticky ends. In: Journal of Physical Chemistry B. 2013 ; Vol. 117, No. 47. pp. 14649-14654.
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