Thin film voltammetry of wild type and mutant reaction center proteins from photosynthetic bacteria

Yun Zhang, Amy M. Lafountain, Nikki Magdaong, Marcel Fuciman, James Paul Allen, Harry A. Frank, James F. Rusling

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Photosynthetic reaction centers (RC) convert light into electrical potential via a series of electron transfers between protein-bound, redox-active cofactors. Direct voltammetry was used to characterize the RC protein from Rhodobacter sphaeroides and mutants with focus on the primary electron donor (P) cofactor. Cyclic voltammetry (CV) and square wave voltammetry (SWV) of lipid and polyion films of RCs revealed similar chemically irreversible processes, and starting, switching, or preconditioning potential of -0.15 V was required to observe a well-defined P/P+ oxidation peak at ∼0.95 V versus normal hydrogen electrode. An irreversible chemical reaction following voltammetric oxidation led to peak decreases upon multiple scans. Mutant RCs with site-directed amino acid modifications in the vicinity of P displayed shifts of oxidation peak potential correlated with those reported from redox titrations. These studies illustrate the utility of thin film voltammetry in characterizing redox properties of bound cofactors in RC proteins.

Original languageEnglish
Pages (from-to)3226-3232
Number of pages7
JournalJournal of Physical Chemistry B
Volume115
Issue number12
DOIs
Publication statusPublished - Mar 31 2011

Fingerprint

Voltammetry
bacteria
Bacteria
proteins
Proteins
Thin films
Oxidation
oxidation
thin films
Photosynthetic Reaction Center Complex Proteins
preconditioning
irreversible processes
Electrons
square waves
Titration
titration
Lipids
Cyclic voltammetry
amino acids
lipids

ASJC Scopus subject areas

  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Physical and Theoretical Chemistry

Cite this

Zhang, Y., Lafountain, A. M., Magdaong, N., Fuciman, M., Allen, J. P., Frank, H. A., & Rusling, J. F. (2011). Thin film voltammetry of wild type and mutant reaction center proteins from photosynthetic bacteria. Journal of Physical Chemistry B, 115(12), 3226-3232. https://doi.org/10.1021/jp111680p

Thin film voltammetry of wild type and mutant reaction center proteins from photosynthetic bacteria. / Zhang, Yun; Lafountain, Amy M.; Magdaong, Nikki; Fuciman, Marcel; Allen, James Paul; Frank, Harry A.; Rusling, James F.

In: Journal of Physical Chemistry B, Vol. 115, No. 12, 31.03.2011, p. 3226-3232.

Research output: Contribution to journalArticle

Zhang, Y, Lafountain, AM, Magdaong, N, Fuciman, M, Allen, JP, Frank, HA & Rusling, JF 2011, 'Thin film voltammetry of wild type and mutant reaction center proteins from photosynthetic bacteria', Journal of Physical Chemistry B, vol. 115, no. 12, pp. 3226-3232. https://doi.org/10.1021/jp111680p
Zhang, Yun ; Lafountain, Amy M. ; Magdaong, Nikki ; Fuciman, Marcel ; Allen, James Paul ; Frank, Harry A. ; Rusling, James F. / Thin film voltammetry of wild type and mutant reaction center proteins from photosynthetic bacteria. In: Journal of Physical Chemistry B. 2011 ; Vol. 115, No. 12. pp. 3226-3232.
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