Third-harmonic microscopy with a titanium-sapphire laser

D. Yelin, Dan Oron, E. Korkotian, M. Segal, Y. Silberberg

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)


We demonstrate third-harmonic microscopy of thin biological objects using a Ti:sapphire laser source. A very simple modification to the collection and detection systems of the microscope allows for the detection of the third-harmonic signal near 270 nm. We show that, using a Ti:sapphire laser, we can combine third-harmonic imaging with two-photon excitation fluorescence simultaneously. Compared to other possible laser sources for third-harmonic microscopy, the Ti:sapphire laser provides better optical resolution, better power availability, and is less absorbed in water. We find that the Ti:sapphire laser is the most suitable laser source for third-harmonic microscopy for thin biological specimens.

Original languageEnglish
JournalApplied Physics B: Lasers and Optics
Issue numberSUPPL.
Publication statusPublished - Jun 2002

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Physics and Astronomy (miscellaneous)

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