Third-harmonic microscopy with a titanium-sapphire laser

D. Yelin, Dan Oron, E. Korkotian, M. Segal, Y. Silberberg

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

We demonstrate third-harmonic microscopy of thin biological objects using a Ti:sapphire laser source. A very simple modification to the collection and detection systems of the microscope allows for the detection of the third-harmonic signal near 270 nm. We show that, using a Ti:sapphire laser, we can combine third-harmonic imaging with two-photon excitation fluorescence simultaneously. Compared to other possible laser sources for third-harmonic microscopy, the Ti:sapphire laser provides better optical resolution, better power availability, and is less absorbed in water. We find that the Ti:sapphire laser is the most suitable laser source for third-harmonic microscopy for thin biological specimens.

Original languageEnglish
JournalApplied Physics B: Lasers and Optics
Volume74
Issue numberSUPPL.
DOIs
Publication statusPublished - Jun 2002

Fingerprint

sapphire
titanium
microscopy
harmonics
lasers
availability
microscopes
fluorescence
photons
water
excitation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Physics and Astronomy (miscellaneous)

Cite this

Third-harmonic microscopy with a titanium-sapphire laser. / Yelin, D.; Oron, Dan; Korkotian, E.; Segal, M.; Silberberg, Y.

In: Applied Physics B: Lasers and Optics, Vol. 74, No. SUPPL., 06.2002.

Research output: Contribution to journalArticle

Yelin, D. ; Oron, Dan ; Korkotian, E. ; Segal, M. ; Silberberg, Y. / Third-harmonic microscopy with a titanium-sapphire laser. In: Applied Physics B: Lasers and Optics. 2002 ; Vol. 74, No. SUPPL.
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