Abstract
We demonstrate third-harmonic microscopy of thin biological objects using a Ti:sapphire laser source. A very simple modification to the collection and detection systems of the microscope allows for the detection of the third-harmonic signal near 270 nm. We show that, using a Ti:sapphire laser, we can combine third-harmonic imaging with two-photon excitation fluorescence simultaneously. Compared to other possible laser sources for third-harmonic microscopy, the Ti:sapphire laser provides better optical resolution, better power availability, and is less absorbed in water. We find that the Ti:sapphire laser is the most suitable laser source for third-harmonic microscopy for thin biological specimens.
| Original language | English |
|---|---|
| Journal | Applied Physics B: Lasers and Optics |
| Volume | 74 |
| Issue number | SUPPL. |
| DOIs | |
| Publication status | Published - Jun 2002 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Physics and Astronomy (miscellaneous)
Cite this
Third-harmonic microscopy with a titanium-sapphire laser. / Yelin, D.; Oron, Dan; Korkotian, E.; Segal, M.; Silberberg, Y.
In: Applied Physics B: Lasers and Optics, Vol. 74, No. SUPPL., 06.2002.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Third-harmonic microscopy with a titanium-sapphire laser
AU - Yelin, D.
AU - Oron, Dan
AU - Korkotian, E.
AU - Segal, M.
AU - Silberberg, Y.
PY - 2002/6
Y1 - 2002/6
N2 - We demonstrate third-harmonic microscopy of thin biological objects using a Ti:sapphire laser source. A very simple modification to the collection and detection systems of the microscope allows for the detection of the third-harmonic signal near 270 nm. We show that, using a Ti:sapphire laser, we can combine third-harmonic imaging with two-photon excitation fluorescence simultaneously. Compared to other possible laser sources for third-harmonic microscopy, the Ti:sapphire laser provides better optical resolution, better power availability, and is less absorbed in water. We find that the Ti:sapphire laser is the most suitable laser source for third-harmonic microscopy for thin biological specimens.
AB - We demonstrate third-harmonic microscopy of thin biological objects using a Ti:sapphire laser source. A very simple modification to the collection and detection systems of the microscope allows for the detection of the third-harmonic signal near 270 nm. We show that, using a Ti:sapphire laser, we can combine third-harmonic imaging with two-photon excitation fluorescence simultaneously. Compared to other possible laser sources for third-harmonic microscopy, the Ti:sapphire laser provides better optical resolution, better power availability, and is less absorbed in water. We find that the Ti:sapphire laser is the most suitable laser source for third-harmonic microscopy for thin biological specimens.
UR - http://www.scopus.com/inward/record.url?scp=0036614712&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036614712&partnerID=8YFLogxK
U2 - 10.1007/s00340-002-0884-x
DO - 10.1007/s00340-002-0884-x
M3 - Article
VL - 74
JO - Applied Physics B: Lasers and Optics
JF - Applied Physics B: Lasers and Optics
SN - 0946-2171
IS - SUPPL.
ER -