Abstract
We demonstrate third-harmonic microscopy of thin biological objects using a Ti:sapphire laser source. A very simple modification to the collection and detection systems of the microscope allows for the detection of the third-harmonic signal near 270 nm. We show that, using a Ti:sapphire laser, we can combine third-harmonic imaging with two-photon excitation fluorescence simultaneously. Compared to other possible laser sources for third-harmonic microscopy, the Ti:sapphire laser provides better optical resolution, better power availability, and is less absorbed in water. We find that the Ti:sapphire laser is the most suitable laser source for third-harmonic microscopy for thin biological specimens.
| Original language | English |
|---|---|
| Journal | Applied Physics B: Lasers and Optics |
| Volume | 74 |
| Issue number | SUPPL. |
| DOIs | |
| Publication status | Published - Jun 2002 |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Physics and Astronomy (miscellaneous)
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Yelin, D., Oron, D., Korkotian, E., Segal, M., & Silberberg, Y. (2002). Third-harmonic microscopy with a titanium-sapphire laser. Applied Physics B: Lasers and Optics, 74(SUPPL.). https://doi.org/10.1007/s00340-002-0884-x