Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein

Jason Tenboer, Shibom Basu, Nadia Zatsepin, Kanupriya Pande, Despina Milathianaki, Matthias Frank, Mark Hunter, Sébastien Boutet, Garth J. Williams, Jason E. Koglin, Dominik Oberthuer, Michael Heymann, Christopher Kupitz, Chelsie Conrad, Jesse Coe, Shatabdi Roy-Chowdhury, Uwe Weierstall, Daniel James, Dingjie Wang, Thomas Grant & 16 others Anton Barty, Oleksandr Yefanov, Jennifer Scales, Cornelius Gati, Carolin Seuring, Vukica Srajer, Robert Henning, Peter Schwander, Raimund Fromme, Abbas Ourmazd, Keith Moffat, Jasper J. Van Thor, John C H Spence, Petra Fromme, Henry N. Chapman, Marius Schmidt

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of biomolecules. We used microcrystals of photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features; these allowed the determination of structures of reaction intermediates to a resolution of 1.6 angstroms. Our results open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.

Original languageEnglish
Pages (from-to)1242-1246
Number of pages5
JournalScience
Volume346
Issue number6214
DOIs
Publication statusPublished - Dec 5 2014

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Crystallography
Electrons
Light
Bacterial Proteins
Proteins
Lasers
X-Rays

ASJC Scopus subject areas

  • General
  • Medicine(all)

Cite this

Tenboer, J., Basu, S., Zatsepin, N., Pande, K., Milathianaki, D., Frank, M., ... Schmidt, M. (2014). Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein. Science, 346(6214), 1242-1246. https://doi.org/10.1126/science.1259357

Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein. / Tenboer, Jason; Basu, Shibom; Zatsepin, Nadia; Pande, Kanupriya; Milathianaki, Despina; Frank, Matthias; Hunter, Mark; Boutet, Sébastien; Williams, Garth J.; Koglin, Jason E.; Oberthuer, Dominik; Heymann, Michael; Kupitz, Christopher; Conrad, Chelsie; Coe, Jesse; Roy-Chowdhury, Shatabdi; Weierstall, Uwe; James, Daniel; Wang, Dingjie; Grant, Thomas; Barty, Anton; Yefanov, Oleksandr; Scales, Jennifer; Gati, Cornelius; Seuring, Carolin; Srajer, Vukica; Henning, Robert; Schwander, Peter; Fromme, Raimund; Ourmazd, Abbas; Moffat, Keith; Van Thor, Jasper J.; Spence, John C H; Fromme, Petra; Chapman, Henry N.; Schmidt, Marius.

In: Science, Vol. 346, No. 6214, 05.12.2014, p. 1242-1246.

Research output: Contribution to journalArticle

Tenboer, J, Basu, S, Zatsepin, N, Pande, K, Milathianaki, D, Frank, M, Hunter, M, Boutet, S, Williams, GJ, Koglin, JE, Oberthuer, D, Heymann, M, Kupitz, C, Conrad, C, Coe, J, Roy-Chowdhury, S, Weierstall, U, James, D, Wang, D, Grant, T, Barty, A, Yefanov, O, Scales, J, Gati, C, Seuring, C, Srajer, V, Henning, R, Schwander, P, Fromme, R, Ourmazd, A, Moffat, K, Van Thor, JJ, Spence, JCH, Fromme, P, Chapman, HN & Schmidt, M 2014, 'Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein', Science, vol. 346, no. 6214, pp. 1242-1246. https://doi.org/10.1126/science.1259357
Tenboer J, Basu S, Zatsepin N, Pande K, Milathianaki D, Frank M et al. Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein. Science. 2014 Dec 5;346(6214):1242-1246. https://doi.org/10.1126/science.1259357
Tenboer, Jason ; Basu, Shibom ; Zatsepin, Nadia ; Pande, Kanupriya ; Milathianaki, Despina ; Frank, Matthias ; Hunter, Mark ; Boutet, Sébastien ; Williams, Garth J. ; Koglin, Jason E. ; Oberthuer, Dominik ; Heymann, Michael ; Kupitz, Christopher ; Conrad, Chelsie ; Coe, Jesse ; Roy-Chowdhury, Shatabdi ; Weierstall, Uwe ; James, Daniel ; Wang, Dingjie ; Grant, Thomas ; Barty, Anton ; Yefanov, Oleksandr ; Scales, Jennifer ; Gati, Cornelius ; Seuring, Carolin ; Srajer, Vukica ; Henning, Robert ; Schwander, Peter ; Fromme, Raimund ; Ourmazd, Abbas ; Moffat, Keith ; Van Thor, Jasper J. ; Spence, John C H ; Fromme, Petra ; Chapman, Henry N. ; Schmidt, Marius. / Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein. In: Science. 2014 ; Vol. 346, No. 6214. pp. 1242-1246.
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