Tip induced fluorescence quenching for nanometer optical and topographical resolution

Olaf Schulz, Zhao Zhao, Alex Ward, Marcelle Koenig, Felix Koberling, Yan Liu, Jörg Enderlein, Hao Yan, Robert Ros

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Progress in nanosciences and life sciences is closely related to developments of high resolution imaging techniques. We introduce a technique which produces correlated topography and fluorescence lifetime images with nanometer resolution. Spot sizes below 5 nm are achieved by quenching of the fluorescence with silicon probes of an atomic force microscope which is combined and synchronized with a confocal fluorescence lifetime microscope. Moreover, we demonstrate the ability to locate and resolve the position of two fluorescent molecules separated by 20.7 nm on a DNA origami triangle with 120 nm side length by correlating topography and fluorescence data. With this method, we anticipate applications in nano- and life sciences, such as the determination of the structure of macromolecular assemblies on surfaces, molecular interactions, as well as the structure and function of nanomaterials.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalOptical Nanoscopy
Volume2
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

quenching
fluorescence
life sciences
topography
microscopes
life (durability)
molecular interactions
triangles
imaging techniques
assemblies
deoxyribonucleic acid
probes
high resolution
silicon
molecules

Keywords

  • Correlated atomic force and fluorescence microscopy
  • Dna nanostructures
  • Optical ultraresolution microscopy
  • Single molecule detection

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Tip induced fluorescence quenching for nanometer optical and topographical resolution. / Schulz, Olaf; Zhao, Zhao; Ward, Alex; Koenig, Marcelle; Koberling, Felix; Liu, Yan; Enderlein, Jörg; Yan, Hao; Ros, Robert.

In: Optical Nanoscopy, Vol. 2, No. 1, 2013, p. 1-8.

Research output: Contribution to journalArticle

Schulz, O, Zhao, Z, Ward, A, Koenig, M, Koberling, F, Liu, Y, Enderlein, J, Yan, H & Ros, R 2013, 'Tip induced fluorescence quenching for nanometer optical and topographical resolution', Optical Nanoscopy, vol. 2, no. 1, pp. 1-8. https://doi.org/10.1186/2192-2853-2-1
Schulz, Olaf ; Zhao, Zhao ; Ward, Alex ; Koenig, Marcelle ; Koberling, Felix ; Liu, Yan ; Enderlein, Jörg ; Yan, Hao ; Ros, Robert. / Tip induced fluorescence quenching for nanometer optical and topographical resolution. In: Optical Nanoscopy. 2013 ; Vol. 2, No. 1. pp. 1-8.
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