Tip induced fluorescence quenching for nanometer optical and topographical resolution

Olaf Schulz, Zhao Zhao, Alex Ward, Marcelle Koenig, Felix Koberling, Yan Liu, Jörg Enderlein, Hao Yan, Robert Ros

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Progress in nanosciences and life sciences is closely related to developments of high resolution imaging techniques. We introduce a technique which produces correlated topography and fluorescence lifetime images with nanometer resolution. Spot sizes below 5 nm are achieved by quenching of the fluorescence with silicon probes of an atomic force microscope which is combined and synchronized with a confocal fluorescence lifetime microscope. Moreover, we demonstrate the ability to locate and resolve the position of two fluorescent molecules separated by 20.7 nm on a DNA origami triangle with 120 nm side length by correlating topography and fluorescence data. With this method, we anticipate applications in nano- and life sciences, such as the determination of the structure of macromolecular assemblies on surfaces, molecular interactions, as well as the structure and function of nanomaterials.

Original languageEnglish
Article number1
Pages (from-to)1-8
Number of pages8
JournalOptical Nanoscopy
Volume2
Issue number1
DOIs
Publication statusPublished - Jan 1 2013

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Keywords

  • Correlated atomic force and fluorescence microscopy
  • Dna nanostructures
  • Optical ultraresolution microscopy
  • Single molecule detection

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Schulz, O., Zhao, Z., Ward, A., Koenig, M., Koberling, F., Liu, Y., Enderlein, J., Yan, H., & Ros, R. (2013). Tip induced fluorescence quenching for nanometer optical and topographical resolution. Optical Nanoscopy, 2(1), 1-8. [1]. https://doi.org/10.1186/2192-2853-2-1