Toward Design Rules for Enzyme Immobilization in Hierarchical Mesoporous Metal-Organic Frameworks

Peng Li, Justin A. Modica, Ashlee J. Howarth, Ernesto Vargas L., Peyman Z. Moghadam, Randall Q. Snurr, Milan Mrksich, Joseph T Hupp, Omar K. Farha

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

The immobilization of enzymes on or in solid supports is crucial for the industrialization of enzymes as chemical catalysts because immobilization provides stabilization, easy separation, and recyclability. Here, we show that a water-stable metal-organic framework, NU-1000, with hierarchical pore structure has the right combination of properties to be particularly well-suited as a scaffold for immobilizing enzymes such that they maintain full enzymatic catalytic activity. The immobilized enzyme shows greater resistance to organic solvent and denaturant than does the free enzyme and is characterized by greater reactant accessibility and higher activity than the same enzyme encapsulated in topologically simpler metal-organic frameworks. These findings suggest design rules for hierarchical pore structuring of host frameworks for enzyme-encapsulation applications by demonstrating enzyme immobilization in a solid support whereby the enzyme is highly accessible and retains catalytic activity under chemically challenging conditions.

Original languageEnglish
Pages (from-to)154-169
Number of pages16
JournalChem
Volume1
Issue number1
DOIs
Publication statusPublished - Dec 1 2016

Fingerprint

Enzyme immobilization
Immobilization
immobilization
Enzymes
Metals
enzyme
metal
Catalyst supports
Catalyst activity
Immobilized Enzymes
Pore structure
Ownership
Encapsulation
Scaffolds
Organic solvents
Scaffolds (biology)
encapsulation
Stabilization
industrialization
accessibility

Keywords

  • SDG3: Good health and well-being

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Biochemistry
  • Environmental Chemistry
  • Materials Chemistry
  • Biochemistry, medical

Cite this

Li, P., Modica, J. A., Howarth, A. J., Vargas L., E., Moghadam, P. Z., Snurr, R. Q., ... Farha, O. K. (2016). Toward Design Rules for Enzyme Immobilization in Hierarchical Mesoporous Metal-Organic Frameworks. Chem, 1(1), 154-169. https://doi.org/10.1016/j.chempr.2016.05.001

Toward Design Rules for Enzyme Immobilization in Hierarchical Mesoporous Metal-Organic Frameworks. / Li, Peng; Modica, Justin A.; Howarth, Ashlee J.; Vargas L., Ernesto; Moghadam, Peyman Z.; Snurr, Randall Q.; Mrksich, Milan; Hupp, Joseph T; Farha, Omar K.

In: Chem, Vol. 1, No. 1, 01.12.2016, p. 154-169.

Research output: Contribution to journalArticle

Li, P, Modica, JA, Howarth, AJ, Vargas L., E, Moghadam, PZ, Snurr, RQ, Mrksich, M, Hupp, JT & Farha, OK 2016, 'Toward Design Rules for Enzyme Immobilization in Hierarchical Mesoporous Metal-Organic Frameworks', Chem, vol. 1, no. 1, pp. 154-169. https://doi.org/10.1016/j.chempr.2016.05.001
Li P, Modica JA, Howarth AJ, Vargas L. E, Moghadam PZ, Snurr RQ et al. Toward Design Rules for Enzyme Immobilization in Hierarchical Mesoporous Metal-Organic Frameworks. Chem. 2016 Dec 1;1(1):154-169. https://doi.org/10.1016/j.chempr.2016.05.001
Li, Peng ; Modica, Justin A. ; Howarth, Ashlee J. ; Vargas L., Ernesto ; Moghadam, Peyman Z. ; Snurr, Randall Q. ; Mrksich, Milan ; Hupp, Joseph T ; Farha, Omar K. / Toward Design Rules for Enzyme Immobilization in Hierarchical Mesoporous Metal-Organic Frameworks. In: Chem. 2016 ; Vol. 1, No. 1. pp. 154-169.
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