Toward point-of-care management of chronic respiratory conditions

Electrochemical sensing of nitrite content in exhaled breath condensate using reduced graphene oxide

Azam Gholizadeh, Damien Voiry, Clifford Weisel, Andrew Gow, Robert Laumbach, Howard Kipen, Manish Chhowalla, Mehdi Javanmard

Research output: Contribution to journalArticle

Abstract

We present a portable non-invasive approach for measuring indicators of inflammation and oxidative stress in the respiratory tract by quantifying a biomarker in exhaled breath condensate (EBC). We discuss the fabrication and characterization of a miniaturized electrochemical sensor for detecting nitrite content in EBC using reduced graphene oxide. The nitrite content in EBC has been demonstrated to be a promising biomarker of inflammation in the respiratory tract, particularly in asthma. We utilized the unique properties of reduced graphene oxide (rGO); specifically, the material is resilient to corrosion while exhibiting rapid electron transfer with electrolytes, thus allowing for highly sensitive electrochemical detection with minimal fouling. Our rGO sensor was housed in an electrochemical cell fabricated from polydimethyl siloxane (PDMS), which was necessary to analyze small EBC sample volumes. The sensor is capable of detecting nitrite at a low over-potential of 0.7 V with respect to an Ag/AgCl reference electrode. We characterized the performance of the sensors using standard nitrite/buffer solutions, nitrite spiked into EBC, and clinical EBC samples. The sensor demonstrated a sensitivity of 0.21 μA μM− 1 cm− 2 in the range of 20–100 μM and of 0.1 μA μM− 1 cm− 2 in the range of 100–1000 μM nitrite concentration and exhibited a low detection limit of 830 nM in the EBC matrix. To benchmark our platform, we tested our sensors using seven pre-characterized clinical EBC samples with concentrations ranging between 0.14 and 6.5 μM. This enzyme-free and label-free method of detecting biomarkers in EBC can pave the way for the development of portable breath analyzers for diagnosing and managing changes in respiratory inflammation and disease.

Original languageEnglish
Article number17022
JournalMicrosystems and Nanoengineering
Volume3
DOIs
Publication statusPublished - Jan 1 2017

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nitrites
Nitrites
Oxides
Graphene
condensates
graphene
Biomarkers
oxides
Sensors
biomarkers
sensors
Electrochemical sensors
Oxidative stress
Electrochemical cells
Siloxanes
Fouling
Labels
asthma
Enzymes

Keywords

  • Electrochemistry
  • Exhaled breath condensate
  • Nitrite
  • Square wave voltammetry
  • Thin-layer-reduced graphene oxide

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Materials Science (miscellaneous)
  • Condensed Matter Physics
  • Industrial and Manufacturing Engineering
  • Electrical and Electronic Engineering

Cite this

Toward point-of-care management of chronic respiratory conditions : Electrochemical sensing of nitrite content in exhaled breath condensate using reduced graphene oxide. / Gholizadeh, Azam; Voiry, Damien; Weisel, Clifford; Gow, Andrew; Laumbach, Robert; Kipen, Howard; Chhowalla, Manish; Javanmard, Mehdi.

In: Microsystems and Nanoengineering, Vol. 3, 17022, 01.01.2017.

Research output: Contribution to journalArticle

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