Toxicity induced enhanced extracellular matrix production in osteoblastic cells cultured on single-walled carbon nanotube networks

Wojtek Tutak, Ki Ho Park, Anatoly Vasilov, Valentin Starovoytov, Giovanni Fanchini, Shi Qing Cai, Nicola C. Partridge, Federico Sesti, Manish Chhowalla

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A central effort in biomedical research concerns the development of materials for sustaining and controlling cell growth. Carbon nanotube based substrates have been shown to support the growth of different kinds of cells (Hu et al 2004 Nano Lett. 4 507-11; Kalbacova et al 2006 Phys. Status Solidi b 13 243; Zanello et al 2006 Nano Lett. 6 562-7); however the underlying molecular mechanisms remain poorly defined. To address the fundamental question of mechanisms by which nanotubes promote bone mitosis and histogenesis, primary calvariae osteoblastic cells were grown on single-walled carbon nanotube thin film (SWNT) substrates. Using a combination of biochemical and optical techniques we demonstrate here that SWNT networks promote cell development through two distinct steps. Initially, SWNTs are absorbed in a process that resembles endocytosis, inducing acute toxicity. Nanotube-mediated cell destruction, however, induces a release of endogenous factors that act to boost the activity of the surviving cells by stimulating the synthesis of extracellular matrix.

Original languageEnglish
Article number255101
JournalNanotechnology
Volume20
Issue number25
DOIs
Publication statusPublished - 2009

Fingerprint

Single-walled carbon nanotubes (SWCN)
Nanotubes
Toxicity
Thin films
Carbon Nanotubes
Cell growth
Substrates
Carbon nanotubes
Bone
Cells

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

Toxicity induced enhanced extracellular matrix production in osteoblastic cells cultured on single-walled carbon nanotube networks. / Tutak, Wojtek; Park, Ki Ho; Vasilov, Anatoly; Starovoytov, Valentin; Fanchini, Giovanni; Cai, Shi Qing; Partridge, Nicola C.; Sesti, Federico; Chhowalla, Manish.

In: Nanotechnology, Vol. 20, No. 25, 255101, 2009.

Research output: Contribution to journalArticle

Tutak, Wojtek ; Park, Ki Ho ; Vasilov, Anatoly ; Starovoytov, Valentin ; Fanchini, Giovanni ; Cai, Shi Qing ; Partridge, Nicola C. ; Sesti, Federico ; Chhowalla, Manish. / Toxicity induced enhanced extracellular matrix production in osteoblastic cells cultured on single-walled carbon nanotube networks. In: Nanotechnology. 2009 ; Vol. 20, No. 25.
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