Toxicity induced enhanced extracellular matrix production in osteoblastic cells cultured on single-walled carbon nanotube networks

Wojtek Tutak, Ki Ho Park, Anatoly Vasilov, Valentin Starovoytov, Giovanni Fanchini, Shi Qing Cai, Nicola C. Partridge, Federico Sesti, Manish Chhowalla

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A central effort in biomedical research concerns the development of materials for sustaining and controlling cell growth. Carbon nanotube based substrates have been shown to support the growth of different kinds of cells (Hu et al 2004 Nano Lett. 4 507-11; Kalbacova et al 2006 Phys. Status Solidi b 13 243; Zanello et al 2006 Nano Lett. 6 562-7); however the underlying molecular mechanisms remain poorly defined. To address the fundamental question of mechanisms by which nanotubes promote bone mitosis and histogenesis, primary calvariae osteoblastic cells were grown on single-walled carbon nanotube thin film (SWNT) substrates. Using a combination of biochemical and optical techniques we demonstrate here that SWNT networks promote cell development through two distinct steps. Initially, SWNTs are absorbed in a process that resembles endocytosis, inducing acute toxicity. Nanotube-mediated cell destruction, however, induces a release of endogenous factors that act to boost the activity of the surviving cells by stimulating the synthesis of extracellular matrix.

Original languageEnglish
Article number255101
JournalNanotechnology
Volume20
Issue number25
DOIs
Publication statusPublished - Jun 29 2009

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Toxicity induced enhanced extracellular matrix production in osteoblastic cells cultured on single-walled carbon nanotube networks'. Together they form a unique fingerprint.

  • Cite this

    Tutak, W., Park, K. H., Vasilov, A., Starovoytov, V., Fanchini, G., Cai, S. Q., Partridge, N. C., Sesti, F., & Chhowalla, M. (2009). Toxicity induced enhanced extracellular matrix production in osteoblastic cells cultured on single-walled carbon nanotube networks. Nanotechnology, 20(25), [255101]. https://doi.org/10.1088/0957-4484/20/25/255101