Toxicological Profiling of Highly Purified Metallic and Semiconducting Single-Walled Carbon Nanotubes in the Rodent Lung and E. coli

Xiang Wang, Nikhita D. Mansukhani, Linda M. Guiney, Jae Hyeok Lee, Ruibin Li, Bingbing Sun, Yu Pei Liao, Chong Hyun Chang, Zhaoxia Ji, Tian Xia, Mark C Hersam, André E. Nel

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The electronic properties of single-walled carbon nanotubes (SWCNTs) are potentially useful for electronics, optics, and sensing applications. Depending on the chirality and diameter, individual SWCNTs can be classified as semiconducting (S-SWCNT) or metallic (M-SWCNT). From a biological perspective, the hazard profiling of purified metallic versus semiconducting SWCNTs has been pursued only in bacteria, with the conclusion that aggregated M-SWCNTs are more damaging to bacterial membranes than S-SWCNTs. However, no comparative studies have been performed in a mammalian system, where most toxicity studies have been undertaken using relatively crude SWCNTs that include a M:S mix at 1:2 ratio. In order to compare the toxicological impact of SWCNTs sorted to enrich them for each of the chirality on pulmonary cells and the intact lung, we used density gradient ultracentrifugation and extensive rinsing to prepare S- and M-SWCNTs that are >98% purified. In vitro screening showed that both tube variants trigger similar amounts of interleukin 1β (IL-1β) and transforming growth factor (TGF-β1) production in THP-1 and BEAS-2B cells, without cytotoxicity. Oropharyngeal aspiration confirmed that both SWCNT variants induce comparable fibrotic effects in the lung and abundance of IL-1β and TGF-β1 release in the bronchoalveolar lavage fluid. There was also no change in the morphology, membrane integrity, and viability of E. coli, in contradistinction to the previously published effects of aggregated tubes on the bacterial membrane. Collectively, these data indicate that the electronic properties and chirality do not independently impact SWCNT toxicological impact in the lung, which is of significance to the safety assessment and incremental use of purified tubes by industry.

Original languageEnglish
Pages (from-to)6008-6019
Number of pages12
JournalACS Nano
Volume10
Issue number6
DOIs
Publication statusPublished - Jun 28 2016

Fingerprint

rodents
Single-walled carbon nanotubes (SWCN)
lungs
Escherichia coli
carbon nanotubes
Chirality
chirality
interleukins
tubes
membranes
Membranes
Interleukin-1
Electronic properties
Rodentia
electronics
Transforming Growth Factors
Cytotoxicity
cells
viability
toxicity

Keywords

  • bacteria
  • electronic property
  • lung toxicity
  • metallic
  • semiconductor
  • SWCNT

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Toxicological Profiling of Highly Purified Metallic and Semiconducting Single-Walled Carbon Nanotubes in the Rodent Lung and E. coli. / Wang, Xiang; Mansukhani, Nikhita D.; Guiney, Linda M.; Lee, Jae Hyeok; Li, Ruibin; Sun, Bingbing; Liao, Yu Pei; Chang, Chong Hyun; Ji, Zhaoxia; Xia, Tian; Hersam, Mark C; Nel, André E.

In: ACS Nano, Vol. 10, No. 6, 28.06.2016, p. 6008-6019.

Research output: Contribution to journalArticle

Wang, X, Mansukhani, ND, Guiney, LM, Lee, JH, Li, R, Sun, B, Liao, YP, Chang, CH, Ji, Z, Xia, T, Hersam, MC & Nel, AE 2016, 'Toxicological Profiling of Highly Purified Metallic and Semiconducting Single-Walled Carbon Nanotubes in the Rodent Lung and E. coli', ACS Nano, vol. 10, no. 6, pp. 6008-6019. https://doi.org/10.1021/acsnano.6b01560
Wang, Xiang ; Mansukhani, Nikhita D. ; Guiney, Linda M. ; Lee, Jae Hyeok ; Li, Ruibin ; Sun, Bingbing ; Liao, Yu Pei ; Chang, Chong Hyun ; Ji, Zhaoxia ; Xia, Tian ; Hersam, Mark C ; Nel, André E. / Toxicological Profiling of Highly Purified Metallic and Semiconducting Single-Walled Carbon Nanotubes in the Rodent Lung and E. coli. In: ACS Nano. 2016 ; Vol. 10, No. 6. pp. 6008-6019.
@article{4685705fde5c496d8701545dd623bd3c,
title = "Toxicological Profiling of Highly Purified Metallic and Semiconducting Single-Walled Carbon Nanotubes in the Rodent Lung and E. coli",
abstract = "The electronic properties of single-walled carbon nanotubes (SWCNTs) are potentially useful for electronics, optics, and sensing applications. Depending on the chirality and diameter, individual SWCNTs can be classified as semiconducting (S-SWCNT) or metallic (M-SWCNT). From a biological perspective, the hazard profiling of purified metallic versus semiconducting SWCNTs has been pursued only in bacteria, with the conclusion that aggregated M-SWCNTs are more damaging to bacterial membranes than S-SWCNTs. However, no comparative studies have been performed in a mammalian system, where most toxicity studies have been undertaken using relatively crude SWCNTs that include a M:S mix at 1:2 ratio. In order to compare the toxicological impact of SWCNTs sorted to enrich them for each of the chirality on pulmonary cells and the intact lung, we used density gradient ultracentrifugation and extensive rinsing to prepare S- and M-SWCNTs that are >98{\%} purified. In vitro screening showed that both tube variants trigger similar amounts of interleukin 1β (IL-1β) and transforming growth factor (TGF-β1) production in THP-1 and BEAS-2B cells, without cytotoxicity. Oropharyngeal aspiration confirmed that both SWCNT variants induce comparable fibrotic effects in the lung and abundance of IL-1β and TGF-β1 release in the bronchoalveolar lavage fluid. There was also no change in the morphology, membrane integrity, and viability of E. coli, in contradistinction to the previously published effects of aggregated tubes on the bacterial membrane. Collectively, these data indicate that the electronic properties and chirality do not independently impact SWCNT toxicological impact in the lung, which is of significance to the safety assessment and incremental use of purified tubes by industry.",
keywords = "bacteria, electronic property, lung toxicity, metallic, semiconductor, SWCNT",
author = "Xiang Wang and Mansukhani, {Nikhita D.} and Guiney, {Linda M.} and Lee, {Jae Hyeok} and Ruibin Li and Bingbing Sun and Liao, {Yu Pei} and Chang, {Chong Hyun} and Zhaoxia Ji and Tian Xia and Hersam, {Mark C} and Nel, {Andr{\'e} E.}",
year = "2016",
month = "6",
day = "28",
doi = "10.1021/acsnano.6b01560",
language = "English",
volume = "10",
pages = "6008--6019",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Toxicological Profiling of Highly Purified Metallic and Semiconducting Single-Walled Carbon Nanotubes in the Rodent Lung and E. coli

AU - Wang, Xiang

AU - Mansukhani, Nikhita D.

AU - Guiney, Linda M.

AU - Lee, Jae Hyeok

AU - Li, Ruibin

AU - Sun, Bingbing

AU - Liao, Yu Pei

AU - Chang, Chong Hyun

AU - Ji, Zhaoxia

AU - Xia, Tian

AU - Hersam, Mark C

AU - Nel, André E.

PY - 2016/6/28

Y1 - 2016/6/28

N2 - The electronic properties of single-walled carbon nanotubes (SWCNTs) are potentially useful for electronics, optics, and sensing applications. Depending on the chirality and diameter, individual SWCNTs can be classified as semiconducting (S-SWCNT) or metallic (M-SWCNT). From a biological perspective, the hazard profiling of purified metallic versus semiconducting SWCNTs has been pursued only in bacteria, with the conclusion that aggregated M-SWCNTs are more damaging to bacterial membranes than S-SWCNTs. However, no comparative studies have been performed in a mammalian system, where most toxicity studies have been undertaken using relatively crude SWCNTs that include a M:S mix at 1:2 ratio. In order to compare the toxicological impact of SWCNTs sorted to enrich them for each of the chirality on pulmonary cells and the intact lung, we used density gradient ultracentrifugation and extensive rinsing to prepare S- and M-SWCNTs that are >98% purified. In vitro screening showed that both tube variants trigger similar amounts of interleukin 1β (IL-1β) and transforming growth factor (TGF-β1) production in THP-1 and BEAS-2B cells, without cytotoxicity. Oropharyngeal aspiration confirmed that both SWCNT variants induce comparable fibrotic effects in the lung and abundance of IL-1β and TGF-β1 release in the bronchoalveolar lavage fluid. There was also no change in the morphology, membrane integrity, and viability of E. coli, in contradistinction to the previously published effects of aggregated tubes on the bacterial membrane. Collectively, these data indicate that the electronic properties and chirality do not independently impact SWCNT toxicological impact in the lung, which is of significance to the safety assessment and incremental use of purified tubes by industry.

AB - The electronic properties of single-walled carbon nanotubes (SWCNTs) are potentially useful for electronics, optics, and sensing applications. Depending on the chirality and diameter, individual SWCNTs can be classified as semiconducting (S-SWCNT) or metallic (M-SWCNT). From a biological perspective, the hazard profiling of purified metallic versus semiconducting SWCNTs has been pursued only in bacteria, with the conclusion that aggregated M-SWCNTs are more damaging to bacterial membranes than S-SWCNTs. However, no comparative studies have been performed in a mammalian system, where most toxicity studies have been undertaken using relatively crude SWCNTs that include a M:S mix at 1:2 ratio. In order to compare the toxicological impact of SWCNTs sorted to enrich them for each of the chirality on pulmonary cells and the intact lung, we used density gradient ultracentrifugation and extensive rinsing to prepare S- and M-SWCNTs that are >98% purified. In vitro screening showed that both tube variants trigger similar amounts of interleukin 1β (IL-1β) and transforming growth factor (TGF-β1) production in THP-1 and BEAS-2B cells, without cytotoxicity. Oropharyngeal aspiration confirmed that both SWCNT variants induce comparable fibrotic effects in the lung and abundance of IL-1β and TGF-β1 release in the bronchoalveolar lavage fluid. There was also no change in the morphology, membrane integrity, and viability of E. coli, in contradistinction to the previously published effects of aggregated tubes on the bacterial membrane. Collectively, these data indicate that the electronic properties and chirality do not independently impact SWCNT toxicological impact in the lung, which is of significance to the safety assessment and incremental use of purified tubes by industry.

KW - bacteria

KW - electronic property

KW - lung toxicity

KW - metallic

KW - semiconductor

KW - SWCNT

UR - http://www.scopus.com/inward/record.url?scp=84976623431&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84976623431&partnerID=8YFLogxK

U2 - 10.1021/acsnano.6b01560

DO - 10.1021/acsnano.6b01560

M3 - Article

VL - 10

SP - 6008

EP - 6019

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 6

ER -