Tunable loading of oligonucleotides with secondary structure on gold nanoparticles through a pH-driven method

Duncan Hieu M Dam, Hyojin Lee, Raymond C. Lee, Ki Hun Kim, Neil L. Kelleher, Teri W Odom

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

This paper describes how pH can be used to control covalent attachment of oligonucleotides with secondary structure on gold nanoparticles (AuNPs). The highest loading of thiolated nucleic acids occurred at low pH (pH = 1.7) due to reduced repulsion between the negatively charged oligonucleotides and the AuNP surface. The packing of oligonucleotides at low pH decreased (single-stranded 蠐 duplex > quadruplex) as the spatial footprint of secondary structure increased. As the pH increased, a decrease in the number of DNA strands grafted to the AuNPs was observed. Notably, the loading density depended on the flexibility and spatial organization of the secondary structures at all pH conditions. At the lowest pH tested, circular dichroism analysis revealed that G-quadruplex aptamers underwent a structural change (from parallel to antiparallel or vice versa), although the biological activity of the aptamer-loaded AuNPs was still maintained. We anticipate that pH-tuning can result in quantitative loading of oligonucleotides on various types of AuNPs with different shapes and surface capping layers.

Original languageEnglish
Pages (from-to)279-285
Number of pages7
JournalBioconjugate Chemistry
Volume26
Issue number2
DOIs
Publication statusPublished - Feb 18 2015

Fingerprint

Oligonucleotides
Gold
Nanoparticles
Nucleic acids
Dichroism
Bioactivity
Nucleic Acids
DNA
Tuning
G-Quadruplexes
Circular Dichroism

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Tunable loading of oligonucleotides with secondary structure on gold nanoparticles through a pH-driven method. / Dam, Duncan Hieu M; Lee, Hyojin; Lee, Raymond C.; Kim, Ki Hun; Kelleher, Neil L.; Odom, Teri W.

In: Bioconjugate Chemistry, Vol. 26, No. 2, 18.02.2015, p. 279-285.

Research output: Contribution to journalArticle

Dam, Duncan Hieu M ; Lee, Hyojin ; Lee, Raymond C. ; Kim, Ki Hun ; Kelleher, Neil L. ; Odom, Teri W. / Tunable loading of oligonucleotides with secondary structure on gold nanoparticles through a pH-driven method. In: Bioconjugate Chemistry. 2015 ; Vol. 26, No. 2. pp. 279-285.
@article{609debd12ec5432e9acfc6d343c27812,
title = "Tunable loading of oligonucleotides with secondary structure on gold nanoparticles through a pH-driven method",
abstract = "This paper describes how pH can be used to control covalent attachment of oligonucleotides with secondary structure on gold nanoparticles (AuNPs). The highest loading of thiolated nucleic acids occurred at low pH (pH = 1.7) due to reduced repulsion between the negatively charged oligonucleotides and the AuNP surface. The packing of oligonucleotides at low pH decreased (single-stranded 蠐 duplex > quadruplex) as the spatial footprint of secondary structure increased. As the pH increased, a decrease in the number of DNA strands grafted to the AuNPs was observed. Notably, the loading density depended on the flexibility and spatial organization of the secondary structures at all pH conditions. At the lowest pH tested, circular dichroism analysis revealed that G-quadruplex aptamers underwent a structural change (from parallel to antiparallel or vice versa), although the biological activity of the aptamer-loaded AuNPs was still maintained. We anticipate that pH-tuning can result in quantitative loading of oligonucleotides on various types of AuNPs with different shapes and surface capping layers.",
author = "Dam, {Duncan Hieu M} and Hyojin Lee and Lee, {Raymond C.} and Kim, {Ki Hun} and Kelleher, {Neil L.} and Odom, {Teri W}",
year = "2015",
month = "2",
day = "18",
doi = "10.1021/bc500562s",
language = "English",
volume = "26",
pages = "279--285",
journal = "Bioconjugate Chemistry",
issn = "1043-1802",
publisher = "American Chemical Society",
number = "2",

}

TY - JOUR

T1 - Tunable loading of oligonucleotides with secondary structure on gold nanoparticles through a pH-driven method

AU - Dam, Duncan Hieu M

AU - Lee, Hyojin

AU - Lee, Raymond C.

AU - Kim, Ki Hun

AU - Kelleher, Neil L.

AU - Odom, Teri W

PY - 2015/2/18

Y1 - 2015/2/18

N2 - This paper describes how pH can be used to control covalent attachment of oligonucleotides with secondary structure on gold nanoparticles (AuNPs). The highest loading of thiolated nucleic acids occurred at low pH (pH = 1.7) due to reduced repulsion between the negatively charged oligonucleotides and the AuNP surface. The packing of oligonucleotides at low pH decreased (single-stranded 蠐 duplex > quadruplex) as the spatial footprint of secondary structure increased. As the pH increased, a decrease in the number of DNA strands grafted to the AuNPs was observed. Notably, the loading density depended on the flexibility and spatial organization of the secondary structures at all pH conditions. At the lowest pH tested, circular dichroism analysis revealed that G-quadruplex aptamers underwent a structural change (from parallel to antiparallel or vice versa), although the biological activity of the aptamer-loaded AuNPs was still maintained. We anticipate that pH-tuning can result in quantitative loading of oligonucleotides on various types of AuNPs with different shapes and surface capping layers.

AB - This paper describes how pH can be used to control covalent attachment of oligonucleotides with secondary structure on gold nanoparticles (AuNPs). The highest loading of thiolated nucleic acids occurred at low pH (pH = 1.7) due to reduced repulsion between the negatively charged oligonucleotides and the AuNP surface. The packing of oligonucleotides at low pH decreased (single-stranded 蠐 duplex > quadruplex) as the spatial footprint of secondary structure increased. As the pH increased, a decrease in the number of DNA strands grafted to the AuNPs was observed. Notably, the loading density depended on the flexibility and spatial organization of the secondary structures at all pH conditions. At the lowest pH tested, circular dichroism analysis revealed that G-quadruplex aptamers underwent a structural change (from parallel to antiparallel or vice versa), although the biological activity of the aptamer-loaded AuNPs was still maintained. We anticipate that pH-tuning can result in quantitative loading of oligonucleotides on various types of AuNPs with different shapes and surface capping layers.

UR - http://www.scopus.com/inward/record.url?scp=84923239508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923239508&partnerID=8YFLogxK

U2 - 10.1021/bc500562s

DO - 10.1021/bc500562s

M3 - Article

VL - 26

SP - 279

EP - 285

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 2

ER -