Tuning the Cavity Size and Chirality of Self-Assembling 3D DNA Crystals

Chad R. Simmons, Fei Zhang, Tara MacCulloch, Noureddine Fahmi, Nicholas Stephanopoulos, Yan Liu, Nadrian C. Seeman, Hao Yan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The foundational goal of structural DNA nanotechnology - the field that uses oligonucleotides as a molecular building block for the programmable self-assembly of nanostructured systems - was to use DNA to construct three-dimensional (3D) lattices for solving macromolecular structures. The programmable nature of DNA makes it an ideal system for rationally constructing self-assembled crystals and immobilizing guest molecules in a repeating 3D array through their specific stereospatial interactions with the scaffold. In this work, we have extended a previously described motif (4 × 5) by expanding the structure to a system that links four double-helical layers; we use a central weaving oligonucleotide containing a sequence of four six-base repeats (4 × 6), forming a matrix of layers that are organized and dictated by a series of Holliday junctions. In addition, we have assembled mirror image crystals (l-DNA) with the identical sequence that are completely resistant to nucleases. Bromine and selenium derivatives were obtained for the l- and d-DNA forms, respectively, allowing phase determination for both forms and solution of the resulting structures to 3.0 and 3.05 Å resolution. Both right- and left-handed forms crystallized in the trigonal space groups with mirror image 3-fold helical screw axes P32 and P31 for each motif, respectively. The structures reveal a highly organized array of discrete and well-defined cavities that are suitable for hosting guest molecules and allow us to dictate a priori the assembly of guest-DNA conjugates with a specified crystalline hand.

Original languageEnglish
Pages (from-to)11254-11260
Number of pages7
JournalJournal of the American Chemical Society
Volume139
Issue number32
DOIs
Publication statusPublished - Aug 16 2017

Fingerprint

Chirality
DNA
Tuning
Crystals
Oligonucleotides
Mirrors
Cruciform DNA
Bromine
Nanotechnology
Molecules
Selenium
Scaffolds
Self assembly
Hand
Crystalline materials
Derivatives

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Tuning the Cavity Size and Chirality of Self-Assembling 3D DNA Crystals. / Simmons, Chad R.; Zhang, Fei; MacCulloch, Tara; Fahmi, Noureddine; Stephanopoulos, Nicholas; Liu, Yan; Seeman, Nadrian C.; Yan, Hao.

In: Journal of the American Chemical Society, Vol. 139, No. 32, 16.08.2017, p. 11254-11260.

Research output: Contribution to journalArticle

Simmons, CR, Zhang, F, MacCulloch, T, Fahmi, N, Stephanopoulos, N, Liu, Y, Seeman, NC & Yan, H 2017, 'Tuning the Cavity Size and Chirality of Self-Assembling 3D DNA Crystals', Journal of the American Chemical Society, vol. 139, no. 32, pp. 11254-11260. https://doi.org/10.1021/jacs.7b06485
Simmons CR, Zhang F, MacCulloch T, Fahmi N, Stephanopoulos N, Liu Y et al. Tuning the Cavity Size and Chirality of Self-Assembling 3D DNA Crystals. Journal of the American Chemical Society. 2017 Aug 16;139(32):11254-11260. https://doi.org/10.1021/jacs.7b06485
Simmons, Chad R. ; Zhang, Fei ; MacCulloch, Tara ; Fahmi, Noureddine ; Stephanopoulos, Nicholas ; Liu, Yan ; Seeman, Nadrian C. ; Yan, Hao. / Tuning the Cavity Size and Chirality of Self-Assembling 3D DNA Crystals. In: Journal of the American Chemical Society. 2017 ; Vol. 139, No. 32. pp. 11254-11260.
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