TY - CHAP
T1 - Two-photon optogenetics
AU - Oron, Dan
AU - Papagiakoumou, Eirini
AU - Anselmi, F.
AU - Emiliani, Valentina
N1 - Funding Information:
D. O. acknowledges support by the European Research Council starting investigator grant SINSLIM and the Crown center of photonics. E. P. was supported by the “Fondation pour la Recherche Médicale” (FRM). F. A. was supported by the European doctoral school Frontières du Vivant. F. A. and V. E. were supported by the “Fondation pour la Recherche Médicale” (FRM équipe) and by the Human Frontier Science Program (RGP0013/2010).
PY - 2012
Y1 - 2012
N2 - The use of optogenetics, the technology that combines genetic and optical methods to monitor and control the activity of specific cell populations, is now widely adopted in neuroscience. The development of optogenetic tools, such as natural photosensitive ion channels and pumps or calcium- and voltage-sensitive proteins, has been growing tremendously during the past 10 years, thanks to the improvement of their performances in terms of facilitating light stimulation. To this aim, efficient illumination methods are also needed. The most common way to photostimulate optogenetic tools has been, so far, widefield illumination with visible light. However, the necessity of addressing the complexity of brain architecture has recently imposed switching to the use of two-photon excitation, which provides a better spatial specificity and deeper penetration in scattering tissue. Two-photon excitation is still challenging, due to intrinsic characteristics of optogenetic tools (e.g., the low conductivity of light-sensitive channels), and efficient illumination methods are therefore essential for advancing in this domain. Here, we present a review on the existing two-photon optical approaches for photoactivation of optogenetic tools, and future perspectives for the widespread implementation of these techniques.
AB - The use of optogenetics, the technology that combines genetic and optical methods to monitor and control the activity of specific cell populations, is now widely adopted in neuroscience. The development of optogenetic tools, such as natural photosensitive ion channels and pumps or calcium- and voltage-sensitive proteins, has been growing tremendously during the past 10 years, thanks to the improvement of their performances in terms of facilitating light stimulation. To this aim, efficient illumination methods are also needed. The most common way to photostimulate optogenetic tools has been, so far, widefield illumination with visible light. However, the necessity of addressing the complexity of brain architecture has recently imposed switching to the use of two-photon excitation, which provides a better spatial specificity and deeper penetration in scattering tissue. Two-photon excitation is still challenging, due to intrinsic characteristics of optogenetic tools (e.g., the low conductivity of light-sensitive channels), and efficient illumination methods are therefore essential for advancing in this domain. Here, we present a review on the existing two-photon optical approaches for photoactivation of optogenetic tools, and future perspectives for the widespread implementation of these techniques.
KW - Digital holography
KW - Generalized phase contrast
KW - Light patterning
KW - Optogenetics
KW - Photoactivation
KW - Temporal focusing
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U2 - 10.1016/B978-0-444-59426-6.00007-0
DO - 10.1016/B978-0-444-59426-6.00007-0
M3 - Chapter
C2 - 22341324
AN - SCOPUS:84857066665
T3 - Progress in Brain Research
SP - 119
EP - 143
BT - Progress in Brain Research
PB - Elsevier B.V.
ER -