Uncoupling Caveolae from Intracellular Signaling in Vivo

Jan R. Kraehling, Zhengrong Hao, Monica Y. Lee, David J. Vinyard, Heino Velazquez, Xinran Liu, Radu V. Stan, Gary W Brudvig, William C. Sessa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Rationale: Caveolin-1 (Cav-1) negatively regulates endothelial nitric oxide (NO) synthase-derived NO production, and this has been mapped to several residues on Cav-1, including F92. Herein, we reasoned that endothelial expression of an F92ACav-1 transgene would let us decipher the mechanisms and relationships between caveolae structure and intracellular signaling. Objective: This study was designed to separate caveolae formation from its downstream signaling effects. Methods and Results: An endothelial-specific doxycycline-regulated mouse model for the expression of Cav-1-F92A was developed. Blood pressure by telemetry and nitric oxide bioavailability by electron paramagnetic resonance and phosphorylation of vasodilator-stimulated phosphoprotein were determined. Caveolae integrity in the presence of Cav-1-F92A was measured by stabilization of caveolin-2, sucrose gradient, and electron microscopy. Histological analysis of heart and lung, echocardiography, and signaling were performed. Conclusions: This study shows that mutant Cav-1-F92A forms caveolae structures similar to WT but leads to increases in NO bioavailability in vivo, thereby demonstrating that caveolae formation and downstream signaling events occur through independent mechanisms.

Original languageEnglish
Pages (from-to)48-55
Number of pages8
JournalCirculation Research
Volume118
Issue number1
DOIs
Publication statusPublished - Jan 8 2016

Fingerprint

Caveolin 1
Caveolae
Nitric Oxide
Biological Availability
Caveolin 2
Telemetry
Doxycycline
Nitric Oxide Synthase Type III
Electron Spin Resonance Spectroscopy
Transgenes
Sucrose
Echocardiography
Electron Microscopy
Phosphorylation
Blood Pressure
Lung

Keywords

  • caveolin-1
  • cell endothelial cell
  • eNOS
  • mice
  • nitric oxide
  • vascular function

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Kraehling, J. R., Hao, Z., Lee, M. Y., Vinyard, D. J., Velazquez, H., Liu, X., ... Sessa, W. C. (2016). Uncoupling Caveolae from Intracellular Signaling in Vivo. Circulation Research, 118(1), 48-55. https://doi.org/10.1161/CIRCRESAHA.115.307767

Uncoupling Caveolae from Intracellular Signaling in Vivo. / Kraehling, Jan R.; Hao, Zhengrong; Lee, Monica Y.; Vinyard, David J.; Velazquez, Heino; Liu, Xinran; Stan, Radu V.; Brudvig, Gary W; Sessa, William C.

In: Circulation Research, Vol. 118, No. 1, 08.01.2016, p. 48-55.

Research output: Contribution to journalArticle

Kraehling, JR, Hao, Z, Lee, MY, Vinyard, DJ, Velazquez, H, Liu, X, Stan, RV, Brudvig, GW & Sessa, WC 2016, 'Uncoupling Caveolae from Intracellular Signaling in Vivo', Circulation Research, vol. 118, no. 1, pp. 48-55. https://doi.org/10.1161/CIRCRESAHA.115.307767
Kraehling JR, Hao Z, Lee MY, Vinyard DJ, Velazquez H, Liu X et al. Uncoupling Caveolae from Intracellular Signaling in Vivo. Circulation Research. 2016 Jan 8;118(1):48-55. https://doi.org/10.1161/CIRCRESAHA.115.307767
Kraehling, Jan R. ; Hao, Zhengrong ; Lee, Monica Y. ; Vinyard, David J. ; Velazquez, Heino ; Liu, Xinran ; Stan, Radu V. ; Brudvig, Gary W ; Sessa, William C. / Uncoupling Caveolae from Intracellular Signaling in Vivo. In: Circulation Research. 2016 ; Vol. 118, No. 1. pp. 48-55.
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