Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model

Joseph F. Georges, Xiaowei Liu, Jennifer Eschbacher, Joshua Nichols, Michael A. Mooney, Anna Joy, Robert F. Spetzler, Burt G. Feuerstein, Trent Anderson, Mark C. Preul, Hao Yan, Peter Nakaji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Improved tools for providing specific intraoperative diagnoses could improve patient care. In neurosurgery, intraoperatively differentiating non-operative lesions can be challenging, often necessitating immunohistochemical (IHC) procedures which require up to 24-48 hours. Here, we evaluate the feasibility of generating rapid ex vivo specific labeling using a novel lymphoma-specific fluorescent switchable aptamer. Our B-cell lymphoma-specific switchable aptamer produced only low-level fluorescence in its unbound conformation and generated an 8-fold increase in fluorescence once bound to its target on CD20-positive lymphoma cells. The aptamer demonstrated strong binding to B-cell lymphoma cells within 10 minutes of incubation. We applied the switchable aptamer to ex vivo xenograft tissue harboring B-cell lymphoma and astrocytoma, and within one hour specific visual identification of lymphoma was routinely possible. In this proof-of-concept study in human cell culture and orthotopic xenografts, we conclude that a fluorescent switchable aptamer can provide rapid and specific labeling of B-cell lymphoma, and that developing aptamer-based labeling approaches could simplify tissue staining and drastically reduce time to histopathological diagnoses compared with IHC-based methods. We propose that switchable aptamers could enhance expeditious, accurate intraoperative decision-making.

Original languageEnglish
Title of host publicationClinical and Translational Neurophotonics 2018
PublisherSPIE
Volume10480
ISBN (Electronic)9781510614451
DOIs
Publication statusPublished - Jan 1 2018
EventClinical and Translational Neurophotonics 2018 - San Francisco, United States
Duration: Jan 27 2018Jan 28 2018

Other

OtherClinical and Translational Neurophotonics 2018
CountryUnited States
CitySan Francisco
Period1/27/181/28/18

Fingerprint

central nervous system
Neurology
B-Cell Lymphoma
Heterografts
Lymphoma
Central Nervous System
Cells
Labeling
marking
Fluorescence
Tissue
Neurosurgery
Astrocytoma
fluorescence
Cell culture
Conformations
decision making
staining
Decision Making
Patient Care

Keywords

  • Aptamer
  • CNS lymphoma
  • Fluorescence imaging
  • Frozen section
  • Glioma
  • In vivo imaging
  • Intraoperative diagnostics
  • Neuro-oncology
  • Stereotactic biopsy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Georges, J. F., Liu, X., Eschbacher, J., Nichols, J., Mooney, M. A., Joy, A., ... Nakaji, P. (2018). Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model. In Clinical and Translational Neurophotonics 2018 (Vol. 10480). [104800G] SPIE. https://doi.org/10.1117/12.2290883

Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model. / Georges, Joseph F.; Liu, Xiaowei; Eschbacher, Jennifer; Nichols, Joshua; Mooney, Michael A.; Joy, Anna; Spetzler, Robert F.; Feuerstein, Burt G.; Anderson, Trent; Preul, Mark C.; Yan, Hao; Nakaji, Peter.

Clinical and Translational Neurophotonics 2018. Vol. 10480 SPIE, 2018. 104800G.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Georges, JF, Liu, X, Eschbacher, J, Nichols, J, Mooney, MA, Joy, A, Spetzler, RF, Feuerstein, BG, Anderson, T, Preul, MC, Yan, H & Nakaji, P 2018, Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model. in Clinical and Translational Neurophotonics 2018. vol. 10480, 104800G, SPIE, Clinical and Translational Neurophotonics 2018, San Francisco, United States, 1/27/18. https://doi.org/10.1117/12.2290883
Georges JF, Liu X, Eschbacher J, Nichols J, Mooney MA, Joy A et al. Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model. In Clinical and Translational Neurophotonics 2018. Vol. 10480. SPIE. 2018. 104800G https://doi.org/10.1117/12.2290883
Georges, Joseph F. ; Liu, Xiaowei ; Eschbacher, Jennifer ; Nichols, Joshua ; Mooney, Michael A. ; Joy, Anna ; Spetzler, Robert F. ; Feuerstein, Burt G. ; Anderson, Trent ; Preul, Mark C. ; Yan, Hao ; Nakaji, Peter. / Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model. Clinical and Translational Neurophotonics 2018. Vol. 10480 SPIE, 2018.
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