Using variable pupil filters to optimize the resolution in multiphoton and saturable fluorescence confocal microscopy

Osip Schwartz, Dan Oron

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13 Citations (Scopus)

Abstract

Pupil filters are widely used to improve the resolution of confocal microscopes. We analyze the possibilities of applying them to N-photon microscopy. We find that taking a linear combination of images obtained with several pupil filters can improve the resolution by a factor of N (compared to a conventional microscope). When applied to saturable fluorescence, this technique allows one to observe fluorescent objects with, in principle, unlimited spatial resolution.

Original languageEnglish
Pages (from-to)464-466
Number of pages3
JournalOptics Letters
Volume34
Issue number4
DOIs
Publication statusPublished - Feb 15 2009

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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