Versatile derivatives of carbohydrate-binding modules for imaging of complex carbohydrates approaching the molecular level of resolution

Shi You Ding, Qi Xu, Mursheda K. Ali, John O. Baker, Edward A. Bayer, Yoav Barak, Raphael Lamed, Junji Sugiyama, Gary Rumbles, Michael E. Himmel

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The innate binding specificity of different carbohydrate-binding modules (CBMs) offers a versatile approach for mapping the chemistry and structure of surfaces that contain complex carbohydrates. We have employed the distinct recognition properties of a double His-tagged recombinant CBM tagged with semiconductor quantum dots for direct imaging of crystalline cellulose at the molecular level of resolution, using transmission and scanning transmission electron microscopy. In addition, three different types of CBMs from families 3, 6, and 20 that exhibit different carbohydrate specificities were each fused with either green fluorescent protein (GFP) or red fluorescent protein (RFP) and employed for double-labeling fluorescence microscopy studies of primary cell walls and various mixtures of complex carbohydrate target molecules. CBM probes can be used for characterizing both native complex carbohydrates and engineered biomaterials.

Original languageEnglish
Pages (from-to)435-443
Number of pages9
JournalBioTechniques
Volume41
Issue number4
DOIs
Publication statusPublished - Oct 2006

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Carbohydrates
Derivatives
Imaging techniques
Quantum Dots
Semiconductors
Scanning Transmission Electron Microscopy
Fluorescence microscopy
Biocompatible Materials
Green Fluorescent Proteins
Complex Mixtures
Fluorescence Microscopy
Cellulose
Cell Wall
Labeling
Semiconductor quantum dots
Cells
Crystalline materials
Transmission electron microscopy
Scanning electron microscopy
Molecules

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Versatile derivatives of carbohydrate-binding modules for imaging of complex carbohydrates approaching the molecular level of resolution. / Ding, Shi You; Xu, Qi; Ali, Mursheda K.; Baker, John O.; Bayer, Edward A.; Barak, Yoav; Lamed, Raphael; Sugiyama, Junji; Rumbles, Gary; Himmel, Michael E.

In: BioTechniques, Vol. 41, No. 4, 10.2006, p. 435-443.

Research output: Contribution to journalArticle

Ding, SY, Xu, Q, Ali, MK, Baker, JO, Bayer, EA, Barak, Y, Lamed, R, Sugiyama, J, Rumbles, G & Himmel, ME 2006, 'Versatile derivatives of carbohydrate-binding modules for imaging of complex carbohydrates approaching the molecular level of resolution', BioTechniques, vol. 41, no. 4, pp. 435-443. https://doi.org/10.2144/000112244
Ding, Shi You ; Xu, Qi ; Ali, Mursheda K. ; Baker, John O. ; Bayer, Edward A. ; Barak, Yoav ; Lamed, Raphael ; Sugiyama, Junji ; Rumbles, Gary ; Himmel, Michael E. / Versatile derivatives of carbohydrate-binding modules for imaging of complex carbohydrates approaching the molecular level of resolution. In: BioTechniques. 2006 ; Vol. 41, No. 4. pp. 435-443.
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