X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC)

J. Domingo Meza-Aguilar, Petra Fromme, Alfredo Torres-Larios, Guillermo Mendoza-Hernández, Ulises Hernandez-Chiñas, Roberto A. Arreguin-Espinosa De Los Monteros, Carlos A. Eslava Campos, Raimund Fromme

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Autotransporters (ATs) represent a superfamily of proteins produced by a variety of pathogenic bacteria, which include the pathogenic groups of Escherichia coli (E. coli) associated with gastrointestinal and urinary tract infections. We present the first X-ray structure of the passenger domain from the Plasmid-encoded toxin (Pet) a 100 kDa protein at 2.3 Å resolution which is a cause of acute diarrhea in both developing and industrialized countries. Pet is a cytoskeleton-altering toxin that induces loss of actin stress fibers. While Pet (pdb code: 4OM9) shows only a sequence identity of 50% compared to the closest related protein sequence, extracellular serine protease plasmid (EspP) the structural features of both proteins are conserved. A closer structural look reveals that Pet contains a β-pleaded sheet at the sequence region of residues 181-190, the corresponding structural domain in EspP consists of a coiled loop. Secondary, the Pet passenger domain features a more pronounced beta sheet between residues 135 and 143 compared to the structure of EspP.

Original languageEnglish
Pages (from-to)439-444
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume445
Issue number2
DOIs
Publication statusPublished - Mar 7 2014

Fingerprint

Enterotoxins
Escherichia coli
Plasmids
Crystal structure
X-Rays
X rays
Serine Proteases
Proteins
Stress Fibers
Type V Secretion Systems
Cytoskeleton
Developed Countries
Urinary Tract Infections
Developing Countries
Gastrointestinal Tract
Actins
Diarrhea
Bacteria
Fibers

Keywords

  • 4OM9
  • Autotransporter
  • EAEC
  • Passenger domain
  • Plasmid encoded toxin(Pet)
  • SPATE

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Domingo Meza-Aguilar, J., Fromme, P., Torres-Larios, A., Mendoza-Hernández, G., Hernandez-Chiñas, U., Arreguin-Espinosa De Los Monteros, R. A., ... Fromme, R. (2014). X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC). Biochemical and Biophysical Research Communications, 445(2), 439-444. https://doi.org/10.1016/j.bbrc.2014.02.016

X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC). / Domingo Meza-Aguilar, J.; Fromme, Petra; Torres-Larios, Alfredo; Mendoza-Hernández, Guillermo; Hernandez-Chiñas, Ulises; Arreguin-Espinosa De Los Monteros, Roberto A.; Eslava Campos, Carlos A.; Fromme, Raimund.

In: Biochemical and Biophysical Research Communications, Vol. 445, No. 2, 07.03.2014, p. 439-444.

Research output: Contribution to journalArticle

Domingo Meza-Aguilar, J, Fromme, P, Torres-Larios, A, Mendoza-Hernández, G, Hernandez-Chiñas, U, Arreguin-Espinosa De Los Monteros, RA, Eslava Campos, CA & Fromme, R 2014, 'X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC)', Biochemical and Biophysical Research Communications, vol. 445, no. 2, pp. 439-444. https://doi.org/10.1016/j.bbrc.2014.02.016
Domingo Meza-Aguilar, J. ; Fromme, Petra ; Torres-Larios, Alfredo ; Mendoza-Hernández, Guillermo ; Hernandez-Chiñas, Ulises ; Arreguin-Espinosa De Los Monteros, Roberto A. ; Eslava Campos, Carlos A. ; Fromme, Raimund. / X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC). In: Biochemical and Biophysical Research Communications. 2014 ; Vol. 445, No. 2. pp. 439-444.
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