X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC)

J. Domingo Meza-Aguilar, Petra Fromme, Alfredo Torres-Larios, Guillermo Mendoza-Hernández, Ulises Hernandez-Chiñas, Roberto A. Arreguin-Espinosa De Los Monteros, Carlos A. Eslava Campos, Raimund Fromme

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Autotransporters (ATs) represent a superfamily of proteins produced by a variety of pathogenic bacteria, which include the pathogenic groups of Escherichia coli (E. coli) associated with gastrointestinal and urinary tract infections. We present the first X-ray structure of the passenger domain from the Plasmid-encoded toxin (Pet) a 100 kDa protein at 2.3 Å resolution which is a cause of acute diarrhea in both developing and industrialized countries. Pet is a cytoskeleton-altering toxin that induces loss of actin stress fibers. While Pet (pdb code: 4OM9) shows only a sequence identity of 50% compared to the closest related protein sequence, extracellular serine protease plasmid (EspP) the structural features of both proteins are conserved. A closer structural look reveals that Pet contains a β-pleaded sheet at the sequence region of residues 181-190, the corresponding structural domain in EspP consists of a coiled loop. Secondary, the Pet passenger domain features a more pronounced beta sheet between residues 135 and 143 compared to the structure of EspP.

Original languageEnglish
Pages (from-to)439-444
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume445
Issue number2
DOIs
Publication statusPublished - Mar 7 2014

Keywords

  • 4OM9
  • Autotransporter
  • EAEC
  • Passenger domain
  • Plasmid encoded toxin(Pet)
  • SPATE

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC)'. Together they form a unique fingerprint.

  • Cite this

    Domingo Meza-Aguilar, J., Fromme, P., Torres-Larios, A., Mendoza-Hernández, G., Hernandez-Chiñas, U., Arreguin-Espinosa De Los Monteros, R. A., Eslava Campos, C. A., & Fromme, R. (2014). X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC). Biochemical and Biophysical Research Communications, 445(2), 439-444. https://doi.org/10.1016/j.bbrc.2014.02.016