X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex

X. Edward Zhou; Xiang Gao; Anton Barty; Yanyong Kang; Yuanzheng He; Wei Liu; Andrii Ishchenko; Thomas A. White; Oleksandr Yefanov; Gye Won Han; Qingping Xu; Parker W. De Waal; Kelly M. Suino-Powell; Sébastien Boutet; Garth J. Williams; Meitian Wang; Dianfan Li; Martin Caffrey; Henry N. Chapman; John C.H. Spence; Petra Fromme; Uwe Weierstall; Raymond C. Stevens; Vadim Cherezov; Karsten Melcher; H. Eric Xu

doi:10.1038/sdata.2016.21

X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex

X. Edward Zhou, Xiang Gao, Anton Barty, Yanyong Kang, Yuanzheng He, Wei Liu, Andrii Ishchenko, Thomas A. White, Oleksandr Yefanov, Gye Won Han, Qingping Xu, Parker W. De Waal, Kelly M. Suino-Powell, Sébastien Boutet, Garth J. Williams, Meitian Wang, Dianfan Li, Martin Caffrey, Henry N. Chapman, John C.H. SpencePetra Fromme, Uwe Weierstall, Raymond C. Stevens, Vadim Cherezov, Karsten Melcher, H. Eric Xu

Arizona State University

Research output: Contribution to journal › Article

29 Citations (Scopus)

Abstract

Serial femtosecond X-ray crystallography (SFX) using an X-ray free electron laser (XFEL) is a recent advancement in structural biology for solving crystal structures of challenging membrane proteins, including G-protein coupled receptors (GPCRs), which often only produce microcrystals. An XFEL delivers highly intense X-ray pulses of femtosecond duration short enough to enable the collection of single diffraction images before significant radiation damage to crystals sets in. Here we report the deposition of the XFEL data and provide further details on crystallization, XFEL data collection and analysis, structure determination, and the validation of the structural model. The rhodopsin-arrestin crystal structure solved with SFX represents the first near-atomic resolution structure of a GPCR-arrestin complex, provides structural insights into understanding of arrestin-mediated GPCR signaling, and demonstrates the great potential of this SFX-XFEL technology for accelerating crystal structure determination of challenging proteins and protein complexes.

Original language	English
Article number	201621
Journal	Scientific Data
Volume	3
DOIs	https://doi.org/10.1038/sdata.2016.21
Publication status	Published - Apr 12 2016

ASJC Scopus subject areas

Statistics and Probability
Information Systems
Education
Computer Science Applications
Statistics, Probability and Uncertainty
Library and Information Sciences

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10.1038/sdata.2016.21

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Zhou, X. E., Gao, X., Barty, A., Kang, Y., He, Y., Liu, W., Ishchenko, A., White, T. A., Yefanov, O., Han, G. W., Xu, Q., De Waal, P. W., Suino-Powell, K. M., Boutet, S., Williams, G. J., Wang, M., Li, D., Caffrey, M., Chapman, H. N., ... Xu, H. E. (2016). X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex. Scientific Data, 3, [201621]. https://doi.org/10.1038/sdata.2016.21

X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex. / Zhou, X. Edward; Gao, Xiang; Barty, Anton; Kang, Yanyong; He, Yuanzheng; Liu, Wei; Ishchenko, Andrii; White, Thomas A.; Yefanov, Oleksandr; Han, Gye Won; Xu, Qingping; De Waal, Parker W.; Suino-Powell, Kelly M.; Boutet, Sébastien; Williams, Garth J.; Wang, Meitian; Li, Dianfan; Caffrey, Martin; Chapman, Henry N.; Spence, John C.H.; Fromme, Petra; Weierstall, Uwe; Stevens, Raymond C.; Cherezov, Vadim; Melcher, Karsten; Xu, H. Eric.

In: Scientific Data, Vol. 3, 201621, 12.04.2016.

Research output: Contribution to journal › Article

Zhou, XE, Gao, X, Barty, A, Kang, Y, He, Y, Liu, W, Ishchenko, A, White, TA, Yefanov, O, Han, GW, Xu, Q, De Waal, PW, Suino-Powell, KM, Boutet, S, Williams, GJ, Wang, M, Li, D, Caffrey, M, Chapman, HN, Spence, JCH, Fromme, P, Weierstall, U, Stevens, RC, Cherezov, V, Melcher, K & Xu, HE 2016, 'X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex', Scientific Data, vol. 3, 201621. https://doi.org/10.1038/sdata.2016.21

Zhou, X. Edward ; Gao, Xiang ; Barty, Anton ; Kang, Yanyong ; He, Yuanzheng ; Liu, Wei ; Ishchenko, Andrii ; White, Thomas A. ; Yefanov, Oleksandr ; Han, Gye Won ; Xu, Qingping ; De Waal, Parker W. ; Suino-Powell, Kelly M. ; Boutet, Sébastien ; Williams, Garth J. ; Wang, Meitian ; Li, Dianfan ; Caffrey, Martin ; Chapman, Henry N. ; Spence, John C.H. ; Fromme, Petra ; Weierstall, Uwe ; Stevens, Raymond C. ; Cherezov, Vadim ; Melcher, Karsten ; Xu, H. Eric. / X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex. In: Scientific Data. 2016 ; Vol. 3.

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abstract = "Serial femtosecond X-ray crystallography (SFX) using an X-ray free electron laser (XFEL) is a recent advancement in structural biology for solving crystal structures of challenging membrane proteins, including G-protein coupled receptors (GPCRs), which often only produce microcrystals. An XFEL delivers highly intense X-ray pulses of femtosecond duration short enough to enable the collection of single diffraction images before significant radiation damage to crystals sets in. Here we report the deposition of the XFEL data and provide further details on crystallization, XFEL data collection and analysis, structure determination, and the validation of the structural model. The rhodopsin-arrestin crystal structure solved with SFX represents the first near-atomic resolution structure of a GPCR-arrestin complex, provides structural insights into understanding of arrestin-mediated GPCR signaling, and demonstrates the great potential of this SFX-XFEL technology for accelerating crystal structure determination of challenging proteins and protein complexes.",

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AU - Ishchenko, Andrii

AU - White, Thomas A.

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AU - Xu, Qingping

AU - De Waal, Parker W.

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AU - Li, Dianfan

AU - Caffrey, Martin

AU - Chapman, Henry N.

AU - Spence, John C.H.

AU - Fromme, Petra

AU - Weierstall, Uwe

AU - Stevens, Raymond C.

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AU - Xu, H. Eric

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