@article{b0b404270e0645acb47efe34c308db76,
title = "X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex",
abstract = "Serial femtosecond X-ray crystallography (SFX) using an X-ray free electron laser (XFEL) is a recent advancement in structural biology for solving crystal structures of challenging membrane proteins, including G-protein coupled receptors (GPCRs), which often only produce microcrystals. An XFEL delivers highly intense X-ray pulses of femtosecond duration short enough to enable the collection of single diffraction images before significant radiation damage to crystals sets in. Here we report the deposition of the XFEL data and provide further details on crystallization, XFEL data collection and analysis, structure determination, and the validation of the structural model. The rhodopsin-arrestin crystal structure solved with SFX represents the first near-atomic resolution structure of a GPCR-arrestin complex, provides structural insights into understanding of arrestin-mediated GPCR signaling, and demonstrates the great potential of this SFX-XFEL technology for accelerating crystal structure determination of challenging proteins and protein complexes.",
author = "Zhou, {X. Edward} and Xiang Gao and Anton Barty and Yanyong Kang and Yuanzheng He and Wei Liu and Andrii Ishchenko and White, {Thomas A.} and Oleksandr Yefanov and Han, {Gye Won} and Qingping Xu and {De Waal}, {Parker W.} and Suino-Powell, {Kelly M.} and S{\'e}bastien Boutet and Williams, {Garth J.} and Meitian Wang and Dianfan Li and Martin Caffrey and Chapman, {Henry N.} and Spence, {John C.H.} and Petra Fromme and Uwe Weierstall and Stevens, {Raymond C.} and Vadim Cherezov and Karsten Melcher and Xu, {H. Eric}",
note = "Funding Information: Portions of this research were carried out at the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory. LCLS is an Office of Science User Facility operated for the U.S. Department of Energy Office of Science by Stanford University. We thank staff members of the Life Science Collaborative Access Team (ID-21) of the Advanced Photon Source (APS) for assistance in data collection at the beam lines of sector 21, which is in part funded by the Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor (Grant 085P1000817), and the General Medicine Collaborative Access Team for assistance in data collection at the beam lines of sector 23 (ID-23), funded in part with Federal funds from the National Cancer Institute (ACB-12002) and the National Institute of General Medical Sciences (AGM-12006). Use of APS was supported by the Office of Science of the US Department of Energy, under Contract No. DE-AC02-06CH11357. This work was supported in part by the Jay and Betty Van Andel Foundation, Ministry of Science and Technology (China) grants 2012ZX09301001 and 2012CB910403, 2013CB910600, XDB08020303, 2013ZX09507001, Amway (China), National Institute of Health grants, DK071662 (H.E.X.); GM102545 and GM104212 (K.M.); the National Institutes of Health Common Fund in Structural Biology grants P50 GM073197 (V.C. and R.C.S.), P50 GM073210 (M.C.), and R01 GM095583 (P.F.); National Institute of General Medical Sciences PSI: Biology grants U54 GM094618 (V.C. and R.C.S.), R01 GM108635 (V.C.), U54 GM094599 (P.F.), GM097463 (J.S.), and U54 GM094586 (JCSG); NSF Science and Technology Center award 1231306 (J.C.H.S., P.F. and U.W); and Science Foundation Ireland, grant 12/IA/1255 (M.C.). Parts of this work were also supported by the Helmholtz Gemeinschaft, the DFG Cluster of Excellence Center for Ultrafast Imaging, and the BMBF project FKZ 05K12CH1 (H.N.C., A.B., O.Y., T.W.). The contributions of E. Boyle-Roden to the 9.7 MAG phase diagram work is gratefully acknowledged. We thank Dr Filipe Maia for his assistance in deposition of the XFEL data to CXIBD.",
year = "2016",
month = apr,
day = "12",
doi = "10.1038/sdata.2016.21",
language = "English",
volume = "3",
journal = "Scientific data",
issn = "2052-4463",
publisher = "Nature Publishing Group",
}