X-ray radiation induces deprotonation of the bilin chromophore in crystalline D. Radiodurans phytochrome

Feifei Li, E. Sethe Burgie, Tao Yu, Annie Héroux, George C Schatz, Richard D. Vierstra, Allen M. Orville

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We report that in the red light-absorbing (Pr) state, the bilin chromophore of the Deinococcus radiodurans proteobacterial phytochrome (DrBphP) is hypersensitive to X-ray photons used in typical synchrotron X-ray protein crystallography experiments. This causes the otherwise fully protonated chromophore to deprotonate without additional major structural changes. These results have major implications for our understanding of the structural and chemical characteristics of the resting and intermediate states of phytochromes and other photoreceptor proteins.

Original languageEnglish
Pages (from-to)2792-2795
Number of pages4
JournalJournal of the American Chemical Society
Volume137
Issue number8
DOIs
Publication statusPublished - Mar 4 2015

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Bile Pigments
Phytochrome
Deprotonation
Chromophores
Deinococcus
X-Rays
Crystalline materials
Proteins
Radiation
X rays
Synchrotrons
Crystallography
X Ray Crystallography
Photons
Light
Experiments

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

X-ray radiation induces deprotonation of the bilin chromophore in crystalline D. Radiodurans phytochrome. / Li, Feifei; Burgie, E. Sethe; Yu, Tao; Héroux, Annie; Schatz, George C; Vierstra, Richard D.; Orville, Allen M.

In: Journal of the American Chemical Society, Vol. 137, No. 8, 04.03.2015, p. 2792-2795.

Research output: Contribution to journalArticle

Li, Feifei ; Burgie, E. Sethe ; Yu, Tao ; Héroux, Annie ; Schatz, George C ; Vierstra, Richard D. ; Orville, Allen M. / X-ray radiation induces deprotonation of the bilin chromophore in crystalline D. Radiodurans phytochrome. In: Journal of the American Chemical Society. 2015 ; Vol. 137, No. 8. pp. 2792-2795.
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