Zwitterion modulation of O2-Evolving activity of cyanobacterial photosystem II

Gözde Ulas, Gary W Brudvig

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Photosystem II (PSII) is the only enzyme in nature that can catalyze the challenging catalytic photooxidation of H2O into four protons, four electrons, and O2. Slowing down turnover of the O2- evolving complex (OEC) is a plausible approach to gain mechanistic information on the reaction. However, modulating the kinetics of the reaction without perturbing the active site is a challenge. In this study, it is shown that the steady-state activity of cyanobacterial PSII is inhibited by small zwitterions, such as glycine betaine and β-alanine. We show that the binding of zwitterions is nondenaturing, is highly reversible, and results in the decrease of the rate of catalytic turnover by ∼50% in the presence of excess zwitterion. Control measurements of photoinduced electron transfer in O 2-inactive PSII show that the inhibition by zwitterions is the result of a specific decrease in the rate of catalytic turnover of the OEC. Recovery of activity upon addition of an exogenous proton carrier (HCO3 -) provides evidence that proton-transfer pathways, thought to be essential for the relay of protons from the OEC to the lumen, are affected. Interestingly, no inhibition is observed for spinach PSII, suggesting that zwitterions act specifically by binding to the extrinsic proteins on the lumenal side of PSII, which differ significantly between plants and cyanobacteria, to slow proton transfer on the electron donor side of PSII.

Original languageEnglish
Pages (from-to)8220-8227
Number of pages8
JournalBiochemistry
Volume49
Issue number37
DOIs
Publication statusPublished - Sep 21 2010

Fingerprint

Photosystem II Protein Complex
Modulation
Protons
Proton transfer
Electrons
Betaine
Spinacia oleracea
Photooxidation
Cyanobacteria
Alanine
Catalytic Domain
Recovery
Kinetics
Enzymes
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)

Cite this

Zwitterion modulation of O2-Evolving activity of cyanobacterial photosystem II. / Ulas, Gözde; Brudvig, Gary W.

In: Biochemistry, Vol. 49, No. 37, 21.09.2010, p. 8220-8227.

Research output: Contribution to journalArticle

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